Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/104284
Title: Reducing intestinal digestion and absorption of fat using a nature-derived biopolymer : interference of triglyceride hydrolysis by nanocellulose
Authors: DeLoid, Glen M.
Sohal, Ikjot Singh
Lorente, Laura R.
Molina, Ramon M.
Pyrgiotakis, Georgios
Stevanovic, Ana
Zhang, Ruojie
McClements, David Julian
Geitner, Nicholas K.
Bousfield, Douglas W.
Demokritou, Philip
Ng, Kee Woei
Loo, Joachim Say Chye
Bell, David C.
Brain, Joseph
Keywords: Engineering::Materials
Nanocellulose
Triglyceride
Issue Date: 2018
Source: DeLoid, G. M., Sohal, I. S., Lorente, L. R., Molina, R. M., Pyrgiotakis, G., Stevanovic, A., … Demokritou, P. (2018). Reducing intestinal digestion and absorption of fat using a nature-derived biopolymer : interference of triglyceride hydrolysis by nanocellulose. ACS Nano, 12(7), 6469-6479. doi:10.1021/acsnano.8b03074
Series/Report no.: ACS Nano
Abstract: Engineered nanomaterials are increasingly added to foods to improve quality, safety, or nutrition. Here we report the ability of ingested nanocellulose (NC) materials to reduce digestion and absorption of ingested fat. In the small intestinal phase of an acellular simulated gastrointestinal tract, the hydrolysis of free fatty acids (FFA) from triglycerides (TG) in a high-fat food model was reduced by 48.4% when NC was added at 0.75% w/w to the food, as quantified by pH stat titration, and by 40.1% as assessed by fluorometric FFA assay. Furthermore, translocation of TG and FFA across an in vitro cellular model of the intestinal epithelium was significantly reduced by the presence of 0.75% w/w NC in the food (TG by 52%, and FFA by 32%). Finally, in in vivo experiments, the postprandial rise in serum TG one hour after gavage with the high fat food model was reduced by 36% when 1.0% w/w NC was administered with the food. As revealed by scanning electron microscopy and molecular dynamics studies, the primary mechanisms for this effect appear to include coalescence of fat droplets on fibrillar NC (CNF) fibers, resulting in a reduction of available surface area for lipase binding, and sequestration of bile salts, causing impaired interfacial displacement of proteins at the lipid droplet surface, and impaired solubilization of lipid digestion products. Together these findings suggest a potential use for NC, as a food additive or supplement, to reduce absorption of ingested fat and thereby assist in weight loss and the management of obesity.
URI: https://hdl.handle.net/10356/104284
http://hdl.handle.net/10220/50214
ISSN: 1936-0851
DOI: 10.1021/acsnano.8b03074
Rights: This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Nano, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acsnano.8b03074
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MSE Journal Articles

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