Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/104313
Title: A Nanoparticle-based Sensor Platform for Cell Tracking and Status/Function Assessment
Authors: Yeo, David
Wiraja, Christian
Chuah, Yon Jin
Gao, Yu
Xu, Chenjie
Issue Date: 2015
Source: Yeo, D., Wiraja, C., Chuah, Y. J., Gao, Y., & Xu, C. (2015). A Nanoparticle-based Sensor Platform for Cell Tracking and Status/Function Assessment. Scientific Reports, 5, 14768-.
Series/Report no.: Scientific Reports
Abstract: Nanoparticles are increasingly popular choices for labeling and tracking cells in biomedical applications such as cell therapy. However, all current types of nanoparticles fail to provide real-time, noninvasive monitoring of cell status and functions while often generating false positive signals. Herein, a nanosensor platform to track the real-time expression of specific biomarkers that correlate with cell status and functions is reported. Nanosensors are synthesized by encapsulating various sensor molecules within biodegradable polymeric nanoparticles. Upon intracellular entry, nanosensors reside within the cell cytoplasm, serving as a depot to continuously release sensor molecules for up to 30 days. In the absence of the target biomarkers, the released sensor molecules remain ‘Off’. When the biomarker(s) is expressed, a detectable signal is generated (On). As a proof-of-concept, three nanosensor formulations were synthesized to monitor cell viability, secretion of nitric oxide, and β-actin mRNA expression.
URI: https://hdl.handle.net/10356/104313
http://hdl.handle.net/10220/38826
ISSN: 2045-2322
DOI: 10.1038/srep14768
Rights: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Journal Articles

Files in This Item:
File Description SizeFormat 
A Nanoparticle-based Sensor Platform for Cell Tracking and Status Function Assessment.pdf2.31 MBAdobe PDFThumbnail
View/Open

SCOPUSTM   
Citations

23
checked on Sep 7, 2020

WEB OF SCIENCETM
Citations

23
checked on Oct 25, 2020

Page view(s)

309
checked on Oct 25, 2020

Download(s)

283
checked on Oct 25, 2020

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.