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Title: Visualization of bone marrow monocyte mobilization using Cx3cr1gfp/+Flt3L-/- reporter mouse by multiphoton intravital microscopy
Authors: Evrard, Maximilien
Chong, Shu Zhen
Devi, Sapna
Chew, Weng Keong
Lee, Bernett
Poidinger, Michael
Ginhoux, Florent
Tan, Suet Mien
Ng, Lai Guan
Keywords: DRNTU::Science::Biological sciences
Issue Date: 2015
Source: Evrard, M., Chong, S. Z., Devi, S., Chew, W. K., Lee, B., Poidinger, M., et al. (2015). Visualization of bone marrow monocyte mobilization using Cx3cr1gfp/+Flt3L-/- reporter mouse by multiphoton intravital microscopy. Journal of leukocyte biology, 97(3), 611-619.
Series/Report no.: Journal of leukocyte biology
Abstract: Monocytes are innate immune cells that play critical roles in inflammation and immune defense. A better comprehension of how monocytes are mobilized and recruited is fundamental to understand their biologic role in disease and steady state. The BM represents a major “checkpoint” for monocyte homeostasis, as it is the primary site for their production and release. Our study determined that the Cx3cr1gfp/+ mouse strain is currently the most ideal model for the visualization of monocyte behavior in the BM by multiphoton intravital microscopy. However, we observed that DCs are also labeled with high levels of GFP and thus, interfere with the accuracy of monocyte tracking in vivo. Hence, we generated a Cx3cr1gfp/+Flt3L−/− reporter mouse and showed that whereas monocyte numbers were not affected, DC numbers were reduced significantly, as DCs but not monocytes depend on Flt3 signaling for their development. We thus verified that mobilization of monocytes from the BM in Cx3cr1gfp/+Flt3L−/− mice is intact in response to LPS. Collectively, our study demonstrates that the Cx3cr1gfp/+Flt3L−/− reporter mouse model represents a powerful tool to visualize monocyte activities in BM and illustrates the potential of a Cx3cr1gfp/+-based, multifunctionality fluorescence reporter approach to dissect monocyte function in vivo.
ISSN: 0741-5400
DOI: 10.1189/jlb.1TA0514-274R
Rights: © 2015 Society for Leukocyte Biology (SLB).
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:SBS Journal Articles

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