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Title: Pushing the envelope in tissue engineering : ex vivo production of thick vascularized cardiac ECM constructs
Authors: Venkatraman, Subbu S.
Machluf, Marcelle
Sarig, Udi
Nguyen, Evelyne Bao-Vi
Wang, Yao
Ting, Sherwin
Bronshtein, Tomer
Sarig, Hadar
Dahan, Nitsan
Gvirtz, Maskit
Reuveny, Shaul
Oh, Steve K.W.
Scheper, Thomas
Boey, Yin Chiang Freddy
Keywords: DRNTU::Science::Medicine::Tissue engineering
Issue Date: 2015
Source: Sarig, U., Nguyen, E. B.-V., Wang, Y., Ting, S., Bronshtein, T., Sarig, H, et al. (2015). Pushing the envelope in tissue engineering: ex vivo production of thick vascularized cardiac ECM constructs. Tissue engineering Part A, 21(9-10), 1507-1519.
Series/Report no.: Tissue engineering Part A
Abstract: Functional vascularization is a prerequisite for cardiac tissue engineering of constructs with physiological thicknesses. We previously reported the successful preservation of main vascular conduits in isolated thick acellular porcine cardiac ventricular ECM (pcECM). We now unveil this scaffold's potential in supporting human cardiomyocytes and promoting new blood vessel development ex vivo, providing long-term cell support in the construct bulk. A custom-designed perfusion bioreactor was developed to remodel such vascularization ex vivo, demonstrating, for the first time, functional angiogenesis in vitro with various stages of vessel maturation supporting up to 1.7 mm thick constructs. A robust methodology was developed to assess the pcECM maximal cell capacity, which resembled the human heart cell density. Taken together these results demonstrate feasibility of producing physiological-like constructs such as the thick pcECM suggested here as a prospective treatment for end-stage heart failure. Methodologies reported herein may also benefit other tissues, offering a valuable in vitro setting for “thick-tissue” engineering strategies toward large animal in vivo studies.
DOI: 10.1089/ten.tea.2014.0477
Schools: School of Materials Science & Engineering 
Rights: © Udi Sarig et al. 2015; Published by Mary Ann Liebert, Inc. This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License ( which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MSE Journal Articles

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