Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/104785
Full metadata record
DC FieldValueLanguage
dc.contributor.authorKalailingam, Pazhanichamyen
dc.contributor.authorTan, Hui Bingen
dc.contributor.authorPan, Jiun Yiten
dc.contributor.authorTan, Suat Hoonen
dc.contributor.authorThanabalu, Thirumaranen
dc.date.accessioned2019-06-11T01:19:54Zen
dc.date.accessioned2019-12-06T21:39:40Z-
dc.date.available2019-06-11T01:19:54Zen
dc.date.available2019-12-06T21:39:40Z-
dc.date.issued2019en
dc.identifier.citationKalailingam, P., Tan, H. B., Pan, J. Y., Tan, S. H., & Thanabalu, T. (2019). Overexpression of CDC42SE1 in A431 cells reduced cell proliferation by inhibiting the Akt pathway. Cells, 8(2), 117-. doi:10.3390/cells8020117en
dc.identifier.issn2073-4409en
dc.identifier.urihttps://hdl.handle.net/10356/104785-
dc.description.abstractCell division cycle 42 (CDC42), a small Rho GTPase, plays a critical role in many cellular processes, including cell proliferation and survival. CDC42 interacts with the CRIB (Cdc42- and Rac-interactive binding) domain of CDC42SE1, a small effector protein of 9 kDa. We found that the expression of CDC42SE1 was reduced in human skin cancer samples relative to matched perilesional control. Exogenous expression of CDC42SE1 but not CDC42SE1H38A (mutation within CRIB domain) in A431 cells (A431SE1, A431SE1-H38A) reduced cell proliferation. Antibody microarray analysis of A431Ctrl and A431SE1 lysate suggested that reduced A431SE1 cells proliferation was due to inhibition of Akt pathway, which was confirmed by the reduced P-Akt and P-mTOR levels in A431SE1 cells compared to A431Ctrl cells. This suggests that CDC42SE1 modulates the CDC42-mediated Akt pathway by competing with other effector proteins to bind CDC42. A431SE1 cells formed smaller colonies in soft agar compared to A431Ctrl and A431SE1-H38A cells. These findings correlate with nude mice xenograft assays, where A431SE1 cells formed tumors with significantly-reduced volume compared to the tumors formed by A431Ctrl cells. Our results suggest that CDC42SE1 is downregulated in skin cancer to promote tumorigenesis, and thus CDC42SE1 might be an important marker of skin cancer progression.en
dc.description.sponsorshipMOE (Min. of Education, S’pore)en
dc.format.extent21 p.en
dc.language.isoenen
dc.relation.ispartofseriesCellsen
dc.rights© 2019 The Author(s). Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).en
dc.subjectCDC42en
dc.subjectSkin Canceren
dc.subjectDRNTU::Science::Biological sciencesen
dc.titleOverexpression of CDC42SE1 in A431 cells reduced cell proliferation by inhibiting the Akt pathwayen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen
dc.identifier.doi10.3390/cells8020117en
dc.description.versionPublished versionen
item.fulltextWith Fulltext-
item.grantfulltextopen-
Appears in Collections:SBS Journal Articles

SCOPUSTM   
Citations 20

7
Updated on Nov 18, 2022

Web of ScienceTM
Citations 20

6
Updated on Nov 26, 2022

Page view(s)

261
Updated on Dec 1, 2022

Download(s) 50

46
Updated on Dec 1, 2022

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.