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Title: Biotin-streptavidin binding interactions of dielectric filled silicon bulk acoustic resonators for smart label-free biochemical sensor applications
Authors: Heidari, Amir
Yoon, Yong-Jin
Park, Woo-Tae
Su, Pei-Chen
Miao, Jianmin
Park, Mi Kyoung
Lin, Julius Tsai Ming
Keywords: DRNTU::Engineering::Mechanical engineering::Bio-mechatronics
Issue Date: 2014
Source: Heidari, A., Yoon, Y.-J., Park, W.-T., Su, P.-C., Miao, J., Lin, J. T. M., et al. (2014). Biotin-Streptavidin Binding Interactions of Dielectric Filled Silicon Bulk Acoustic Resonators for Smart Label-Free Biochemical Sensor Applications. Sensors, 14(3), 4585-4598.
Series/Report no.: Sensors
Abstract: Sensor performance of a dielectric filled silicon bulk acoustic resonator type label-free biosensor is verified with biotin-streptavidin binding interactions as a model system. The mass sensor is a micromachined silicon square plate with a dielectric filled capacitive excitation mechanism. The resonance frequency of the biotin modified resonator decreased 315 ppm when exposed to streptavidin solution for 15 min with a concentration of 10−7 M, corresponding to an added mass of 3.43 ng on the resonator surface. An additional control is added by exposing a bovine serum albumin (BSA)-covered device to streptavidin in the absence of the attached biotin. No resonance frequency shift was observed in the control experiment, which confirms the specificity of the detection. The sensor-to-sensor variability is also measured to be 4.3%. Consequently, the developed sensor can be used to observe in biotin-streptavidin interaction without the use of labelling or molecular tags. In addition, biosensor can be used in a variety of different immunoassay tests.
ISSN: 1424-8220
DOI: 10.3390/s140304585
Rights: © 2014 by the authors; licensee MDPI, Basel, Switzerland. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MAE Journal Articles

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