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dc.contributor.authorSchuster, Stephan Christophen
dc.contributor.authorWang, Tingtingen
dc.contributor.authorCai, Zhaoen
dc.contributor.authorShao, Xiaolongen
dc.contributor.authorZhang, Weitongen
dc.contributor.authorXie, Yingpengen
dc.contributor.authorZhang, Yingchaoen
dc.contributor.authorHua, Canfengen
dc.contributor.authorYang, Liangen
dc.contributor.authorDeng, Xinen
dc.contributor.editorMcBain, Andrew J.en
dc.identifier.citationWang, T., Cai, Z., Shao, X., Zhang, W., Xie, Y., Zhang, Y., . . . Deng, X. (2019). Pleiotropic effects of c-di-GMP content in Pseudomonas syringae. Applied and Environmental Microbiology, 85(10), e00152-19-. doi:10.1128/AEM.00152-19en
dc.description.abstractAlthough the ubiquitous bacterial secondary messenger cyclic diguanylate (c-di-GMP) has important cellular functions in a wide range of bacteria, its function in the model plant pathogen Pseudomonas syringae remains largely elusive. To this end, we overexpressed Escherichia coli diguanylate cyclase (YedQ) and phosphodiesterase (YhjH) in P. syringae, resulting in high and low in vivo levels of c-di-GMP, respectively. Via genome-wide RNA sequencing of these two strains, we found that c-di-GMP regulates (i) fliN, fliE, and flhA, which are associated with flagellar assembly; (ii) alg8 and alg44, which are related to the exopolysaccharide biosynthesis pathway; (iii) pvdE, pvdP, and pvsA, which are associated with the siderophore biosynthesis pathway; and (iv) sodA, which encodes a superoxide dismutase. In particular, we identified three promoters that are sensitive to elevated levels of c-di-GMP and inserted them into luciferase-based reporters that respond effectively to the c-di-GMP levels in P. syringae; these promoters could be useful in the measurement of in vivo levels of c-di-GMP in real time. Further phenotypic assays validated the RNA sequencing (RNA-seq) results and confirmed the effect on c-di-GMP-associated pathways, such as repressing the type III secretion system (T3SS) and motility while inducing biofilm production, siderophore production, and oxidative stress resistance. Taken together, these results demonstrate that c-di-GMP regulates the virulence and stress response in P. syringae, which suggests that tuning its level could be a new strategy to protect plants from attacks by this pathogen.en
dc.description.sponsorshipNMRC (Natl Medical Research Council, S’pore)en
dc.format.extent15 p.en
dc.relation.ispartofseriesApplied and Environmental Microbiologyen
dc.rights© 2019 Wang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.en
dc.subjectDRNTU::Science::Biological sciencesen
dc.subjectPseudomonas Syringaeen
dc.titlePleiotropic effects of c-di-GMP content in Pseudomonas syringaeen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen
dc.contributor.researchSingapore Centre for Environmental Life Sciences and Engineeringen
dc.description.versionPublished versionen
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