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Title: Pleiotropic effects of c-di-GMP content in Pseudomonas syringae
Authors: Schuster, Stephan Christoph
Wang, Tingting
Cai, Zhao
Shao, Xiaolong
Zhang, Weitong
Xie, Yingpeng
Zhang, Yingchao
Hua, Canfeng
Yang, Liang
Deng, Xin
Keywords: DRNTU::Science::Biological sciences
Pseudomonas Syringae
Issue Date: 2019
Source: Wang, T., Cai, Z., Shao, X., Zhang, W., Xie, Y., Zhang, Y., . . . Deng, X. (2019). Pleiotropic effects of c-di-GMP content in Pseudomonas syringae. Applied and Environmental Microbiology, 85(10), e00152-19-. doi:10.1128/AEM.00152-19
Series/Report no.: Applied and Environmental Microbiology
Abstract: Although the ubiquitous bacterial secondary messenger cyclic diguanylate (c-di-GMP) has important cellular functions in a wide range of bacteria, its function in the model plant pathogen Pseudomonas syringae remains largely elusive. To this end, we overexpressed Escherichia coli diguanylate cyclase (YedQ) and phosphodiesterase (YhjH) in P. syringae, resulting in high and low in vivo levels of c-di-GMP, respectively. Via genome-wide RNA sequencing of these two strains, we found that c-di-GMP regulates (i) fliN, fliE, and flhA, which are associated with flagellar assembly; (ii) alg8 and alg44, which are related to the exopolysaccharide biosynthesis pathway; (iii) pvdE, pvdP, and pvsA, which are associated with the siderophore biosynthesis pathway; and (iv) sodA, which encodes a superoxide dismutase. In particular, we identified three promoters that are sensitive to elevated levels of c-di-GMP and inserted them into luciferase-based reporters that respond effectively to the c-di-GMP levels in P. syringae; these promoters could be useful in the measurement of in vivo levels of c-di-GMP in real time. Further phenotypic assays validated the RNA sequencing (RNA-seq) results and confirmed the effect on c-di-GMP-associated pathways, such as repressing the type III secretion system (T3SS) and motility while inducing biofilm production, siderophore production, and oxidative stress resistance. Taken together, these results demonstrate that c-di-GMP regulates the virulence and stress response in P. syringae, which suggests that tuning its level could be a new strategy to protect plants from attacks by this pathogen.
ISSN: 0099-2240
DOI: 10.1128/AEM.00152-19
Rights: © 2019 Wang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles
SCELSE Journal Articles

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