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Title: Chromatin laser imaging reveals abnormal nuclear changes for early cancer detection
Authors: Chen, Yu-Cheng
Chen, Qiushu
Tan, Xiaotain
Chen, Grace
Bergin, Ingrid
Muhammad Nadeem Aslam
Fan, Xudong
Keywords: DRNTU::Engineering::Electrical and electronic engineering
Laser-emission Microscopy
Issue Date: 2019
Source: Chen, Y.-C., Chen, Q., Tan, X., Chen, G., Bergin, I., Muhammad Nadeem Aslam., & Fan, X. (2019). Chromatin laser imaging reveals abnormal nuclear changes for early cancer detection. Biomedical Optics Express, 10(2), 838-854. doi:10.1364/BOE.10.000838
Series/Report no.: Biomedical Optics Express
Abstract: We developed and applied rapid scanning laser-emission microscopy (LEM) to detect abnormal changes in cell nuclei for early diagnosis of cancer and cancer precursors. Regulation of chromatins is essential for genetic development and normal cell functions, while abnormal nuclear changes may lead to many diseases, in particular, cancer. The capability to detect abnormal changes in “apparently normal” tissues at a stage earlier than tumor development is critical for cancer prevention. Here we report using LEM to analyze colonic tissues from mice at-risk for colon cancer (induced by a high-fat diet) by detecting pre-polyp nuclear abnormality. By imaging the lasing emissions from chromatins, we discovered that, despite the absence of observable lesions, polyps, or tumors under stereoscope, high-fat mice exhibited significantly lower lasing thresholds than low-fat mice. The low lasing threshold is, in fact, very similar to that of adenomas and is caused by abnormal cell proliferation and chromatin deregulation that can potentially lead to cancer. Our findings suggest that conventional detection methods, such as colonoscopy followed by histopathology, by itself, may be insufficient to reveal hidden or early tumors under development. We envision that this innovative work will provide new insights into LEM and support existing tools for early tumor detection in clinical diagnosis, and fundamental biological and biomedical research of chromatin changes at the biomolecular level of cancer development.
DOI: 10.1364/BOE.10.000838
Rights: © 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:EEE Journal Articles

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