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dc.contributor.authorOng, Seow Thengen
dc.contributor.authorFreeley, Michaelen
dc.contributor.authorSkubis-Zegadło, Joannaen
dc.contributor.authorFazil, Mobashar Hussain Urf Turabeen
dc.contributor.authorKelleher, Dermoten
dc.contributor.authorFresser, Friedrichen
dc.contributor.authorBaier, Gottfrieden
dc.contributor.authorVerma, Navin Kumaren
dc.contributor.authorLong, Aideenen
dc.identifier.citationOng, S. T., Freeley, M., Skubis-Zegadło, J., Fazil, M. H. U. T., Kelleher, D., Fresser, F., et al. (2014). Phosphorylation of Rab5a protein by protein kinase Cϵ is crucial for T-cell migration. Journal of biological chemistry, 289(28), 19420-19434.en
dc.description.abstractRab GTPases control membrane traffic and receptor-mediated endocytosis. Within this context, Rab5a plays an important role in the spatial regulation of intracellular transport and signal transduction processes. Here, we report a previously uncharacterized role for Rab5a in the regulation of T-cell motility. We show that Rab5a physically associates with protein kinase C ε (PKC ε ) in migrating T-cells. Following stimulation of T- cells through the integrin LFA-1 or the chemokine receptor CXCR4, Rab5a is phosphorylated on a N-terminal T7 site by PKC ε . Both Rab5a and PKC ε dynamically interact at the centrosomal region of migrating cells, and PKC ε -mediated phosphorylation on T7 regulates Rab5a trafficking to the cell leading edge. Further, we demonstrate that Rab5a T7 phosphorylation is functionally necessary for Rac1 activation, actin rearrangement and T- cell motility. We present a novel mechanism by which a PKC ε -Rab5a-Rac1 axis regulates cytoskeleton remodelling and T-cell migration, both of which are central for the adaptive immune response.en
dc.format.extent25 p.en
dc.relation.ispartofseriesJournal of biological chemistryen
dc.rights© 2014 by The American Society for Biochemistry and Molecular Biology, Inc. This is the author created version of a work that has been peer reviewed and accepted for publication by Journal of Biological Chemistry, The American Society for Biochemistry and Molecular Biology, Inc. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [].en
dc.subjectDRNTU::Science::Biological sciencesen
dc.titlePhosphorylation of Rab5a protein by protein kinase Cϵ is crucial for T-cell migrationen
dc.typeJournal Articleen
dc.contributor.schoolLee Kong Chian School of Medicine (LKCMedicine)en
dc.description.versionAccepted versionen
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