Please use this identifier to cite or link to this item:
https://hdl.handle.net/10356/105965
Title: | SSRP1 promotes colorectal cancer progression and is negatively regulated by miR‐28‐5p | Authors: | Deng, Yunchao Luo, Xu Yu, Honggang Ding, Qianshan Xiang, Guoan Wu, Wei He, Ke Guo, Qian Chen, Jingdi Zhang, Mengjiao Huang, Kai Yang, Dongmei Wu, Lu |
Keywords: | MicroRNA Colorectal Cancer DRNTU::Engineering::Electrical and electronic engineering |
Issue Date: | 2019 | Source: | Wu, W., He, K., Guo, Q., Chen, J., Zhang, M., Huang, K., . . . Xiang, G. (2019). SSRP1 promotes colorectal cancer progression and is negatively regulated by miR‐28‐5p. Journal of Cellular and Molecular Medicine, 23(5), 3118-3129. doi:10.1111/jcmm.14134 | Series/Report no.: | Journal of Cellular and Molecular Medicine | Abstract: | In this study, microarray data analysis, real‐time quantitative PCR and immunohistochemistry were used to detect the expression levels of SSRP1 in colorectal cancer (CRC) tissue and in corresponding normal tissue. The association between structure‐specific recognition protein 1 (SSRP1) expression and patient prognosis was examined by Kaplan‐Meier analysis. SSRP1 was knocked down and overexpressed in CRC cell lines, and its effects on proliferation, cell cycling, migration, invasion, cellular energy metabolism, apoptosis, chemotherapeutic drug sensitivity and cell phenotype‐related molecules were assessed. The growth of xenograft tumours in nude mice was also assessed. MiRNAs that potentially targeted SSRP1 were determined by bioinformatic analysis, Western blotting and luciferase reporter assays. We showed that SSRP1 mRNA levels were significantly increased in CRC tissue. We also confirmed that this upregulation was related to the terminal tumour stage in CRC patients, and high expression levels of SSRP1 predicted shorter disease‐free survival and faster relapse. We also found that SSRP1 modulated proliferation, metastasis, cellular energy metabolism and the epithelial‐mesenchymal transition in CRC. Furthermore, SSRP1 induced apoptosis and SSRP1 knockdown augmented the sensitivity of CRC cells to 5‐fluorouracil and cisplatin. Moreover, we explored the molecular mechanisms accounting for the dysregulation of SSRP1 in CRC and identified microRNA‐28‐5p (miR‐28‐5p) as a direct upstream regulator of SSRP1. We concluded that SSRP1 promotes CRC progression and is negatively regulated by miR‐28‐5p. | URI: | https://hdl.handle.net/10356/105965 http://hdl.handle.net/10220/48804 |
ISSN: | 1582-1838 | DOI: | 10.1111/jcmm.14134 | Rights: | © 2019 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. | Fulltext Permission: | open | Fulltext Availability: | With Fulltext |
Appears in Collections: | EEE Journal Articles |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
SSRP1 promotes colorectal cancer progression and is negatively regulated by miR‐2.pdf | 2.17 MB | Adobe PDF | ![]() View/Open |
SCOPUSTM
Citations
20
9
Updated on Jul 16, 2020
PublonsTM
Citations
20
14
Updated on Mar 4, 2021
Page view(s)
272
Updated on Aug 14, 2022
Download(s) 50
46
Updated on Aug 14, 2022
Google ScholarTM
Check
Altmetric
Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.