Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/105967
Title: Integrated genomic and metabolomic approach to the discovery of potential anti-quorum sensing natural products from microbes associated with marine samples from Singapore
Authors: Chin, Joyce Seow Fong
Tan, Koh Siang
Goh, Hui Chin
Lim, Swee Cheng
Pang, Li Mei
Gerwick, William H.
Tan, Lik Tong
Liang, Zhao-Xun
Yang, Liang
Glukhov, Evgenia
Ong, Marshall Ji Fa
Keywords: Marine Bacteria
DRNTU::Science::Biological sciences
Marine Sponges
Issue Date: 2019
Source: Ong, M. J. F., Goh, H. C., Lim, S. C., Pang, L. M., Chin, J. S. F., Tan, K. S., . . . Tan, L. T. (2019). Integrated genomic and metabolomic approach to the discovery of potential anti-quorum sensing natural products from microbes associated with marine samples from Singapore. Marine Drugs, 17(1), 72-. doi:10.3390/md17010072
Series/Report no.: Marine Drugs
Abstract: With 70% of the Earth’s surface covered in water, the marine ecosystem offers immense opportunities for drug discovery and development. Due to the decreasing rate of novel natural product discovery from terrestrial sources in recent years, many researchers are beginning to look seaward for breakthroughs in new therapeutic agents. As part of an ongoing marine drug discovery programme in Singapore, an integrated approach of combining metabolomic and genomic techniques were initiated for uncovering novel anti-quorum sensing molecules from bacteria associated with subtidal samples collected in the Singapore Strait. Based on the culture-dependent method, a total of 102 marine bacteria strains were isolated and the identities of selected strains were established based on their 16S rRNA gene sequences. About 5% of the marine bacterial organic extracts showed quorum sensing inhibitory (QSI) activity in a dose-dependent manner based on the Pseudomonas aeruginosa QS reporter system. In addition, the extracts were subjected to mass spectrometry-based molecular networking and the genome of selected strains were analysed for known as well as new biosynthetic gene clusters. This study revealed that using integrated techniques, coupled with biological assays, can provide an effective and rapid prioritization of marine bacterial strains for downstream large-scale culturing for the purpose of isolation and structural elucidation of novel bioactive compounds.
URI: https://hdl.handle.net/10356/105967
http://hdl.handle.net/10220/48802
ISSN: 1660-3397
DOI: 10.3390/md17010072
Rights: © 2019 The Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles
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