Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/105967
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dc.contributor.authorChin, Joyce Seow Fongen
dc.contributor.authorTan, Koh Siangen
dc.contributor.authorGoh, Hui Chinen
dc.contributor.authorLim, Swee Chengen
dc.contributor.authorPang, Li Meien
dc.contributor.authorGerwick, William H.en
dc.contributor.authorTan, Lik Tongen
dc.contributor.authorLiang, Zhao-Xunen
dc.contributor.authorYang, Liangen
dc.contributor.authorGlukhov, Evgeniaen
dc.contributor.authorOng, Marshall Ji Faen
dc.date.accessioned2019-06-18T07:21:02Zen
dc.date.accessioned2019-12-06T22:01:49Z-
dc.date.available2019-06-18T07:21:02Zen
dc.date.available2019-12-06T22:01:49Z-
dc.date.issued2019en
dc.identifier.citationOng, M. J. F., Goh, H. C., Lim, S. C., Pang, L. M., Chin, J. S. F., Tan, K. S., . . . Tan, L. T. (2019). Integrated genomic and metabolomic approach to the discovery of potential anti-quorum sensing natural products from microbes associated with marine samples from Singapore. Marine Drugs, 17(1), 72-. doi:10.3390/md17010072en
dc.identifier.issn1660-3397en
dc.identifier.urihttps://hdl.handle.net/10356/105967-
dc.description.abstractWith 70% of the Earth’s surface covered in water, the marine ecosystem offers immense opportunities for drug discovery and development. Due to the decreasing rate of novel natural product discovery from terrestrial sources in recent years, many researchers are beginning to look seaward for breakthroughs in new therapeutic agents. As part of an ongoing marine drug discovery programme in Singapore, an integrated approach of combining metabolomic and genomic techniques were initiated for uncovering novel anti-quorum sensing molecules from bacteria associated with subtidal samples collected in the Singapore Strait. Based on the culture-dependent method, a total of 102 marine bacteria strains were isolated and the identities of selected strains were established based on their 16S rRNA gene sequences. About 5% of the marine bacterial organic extracts showed quorum sensing inhibitory (QSI) activity in a dose-dependent manner based on the Pseudomonas aeruginosa QS reporter system. In addition, the extracts were subjected to mass spectrometry-based molecular networking and the genome of selected strains were analysed for known as well as new biosynthetic gene clusters. This study revealed that using integrated techniques, coupled with biological assays, can provide an effective and rapid prioritization of marine bacterial strains for downstream large-scale culturing for the purpose of isolation and structural elucidation of novel bioactive compounds.en
dc.description.sponsorshipNRF (Natl Research Foundation, S’pore)en
dc.format.extent15 p.en
dc.language.isoenen
dc.relation.ispartofseriesMarine Drugsen
dc.rights© 2019 The Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).en
dc.subjectMarine Bacteriaen
dc.subjectDRNTU::Science::Biological sciencesen
dc.subjectMarine Spongesen
dc.titleIntegrated genomic and metabolomic approach to the discovery of potential anti-quorum sensing natural products from microbes associated with marine samples from Singaporeen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen
dc.contributor.schoolNational Institute of Educationen
dc.contributor.researchSingapore Centre for Environmental Life Sciences and Engineeringen
dc.identifier.doi10.3390/md17010072en
dc.description.versionPublished versionen
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item.grantfulltextopen-
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