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|Title:||Biomimetic production of silk-like recombinant squid sucker ring teeth proteins||Authors:||Ding, Dawei
Guerette, Paul A.
Kong, Kiat Whye
|Keywords:||DRNTU::Science::Biological sciences::Molecular biology||Issue Date:||2014||Source:||Ding, D., Guerette, P. A., Hoon, S., Kong, K. W., Cornvik, T., Nilsson, M., et al. (2014). Biomimetic production of silk-like recombinant squid sucker ring teeth proteins. Biomacromolecules, 15(9), 3278-3289.||Series/Report no.:||Biomacromolecules||Abstract:||The sucker ring teeth (SRT) of Humboldt squid exhibit mechanical properties that rival those of robust engineered synthetic polymers. Remarkably, these properties are achieved without a mineral phase or covalent cross-links. Instead, SRT are exclusively made of silk-like proteins called “suckerins”, which assemble into nanoconfined β-sheet reinforced supramolecular networks. In this study, three streamlined strategies for full-length recombinant suckerin protein production and purification were developed. Recombinant suckerin exhibited high solubility and colloidal stability in aqueous-based solvents. In addition, the colloidal suspensions exhibited a concentration-dependent conformational switch, from random coil to β-sheet enriched structures. Our results demonstrate that recombinant suckerin can be produced in a facile manner in E. coli and processed from mild aqueous solutions into materials enriched in β-sheets. We suggest that recombinant suckerin-based materials offer potential for a range of biomedical and engineering applications.||URI:||https://hdl.handle.net/10356/106185
|ISSN:||1525-7797||DOI:||10.1021/bm500670r||Rights:||© 2014 American Chemical Society.||Fulltext Permission:||none||Fulltext Availability:||No Fulltext|
|Appears in Collections:||ERI@N Journal Articles|
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