Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/106185
Title: Biomimetic production of silk-like recombinant squid sucker ring teeth proteins
Authors: Ding, Dawei
Guerette, Paul A.
Hoon, Shawn
Kong, Kiat Whye
Cornvik, Tobias
Nilsson, Martina
Kumar, Akshita
Lescar, Julien
Miserez, Ali
Keywords: DRNTU::Science::Biological sciences::Molecular biology
Issue Date: 2014
Source: Ding, D., Guerette, P. A., Hoon, S., Kong, K. W., Cornvik, T., Nilsson, M., et al. (2014). Biomimetic production of silk-like recombinant squid sucker ring teeth proteins. Biomacromolecules, 15(9), 3278-3289.
Series/Report no.: Biomacromolecules
Abstract: The sucker ring teeth (SRT) of Humboldt squid exhibit mechanical properties that rival those of robust engineered synthetic polymers. Remarkably, these properties are achieved without a mineral phase or covalent cross-links. Instead, SRT are exclusively made of silk-like proteins called “suckerins”, which assemble into nanoconfined β-sheet reinforced supramolecular networks. In this study, three streamlined strategies for full-length recombinant suckerin protein production and purification were developed. Recombinant suckerin exhibited high solubility and colloidal stability in aqueous-based solvents. In addition, the colloidal suspensions exhibited a concentration-dependent conformational switch, from random coil to β-sheet enriched structures. Our results demonstrate that recombinant suckerin can be produced in a facile manner in E. coli and processed from mild aqueous solutions into materials enriched in β-sheets. We suggest that recombinant suckerin-based materials offer potential for a range of biomedical and engineering applications.
URI: https://hdl.handle.net/10356/106185
http://hdl.handle.net/10220/24460
ISSN: 1525-7797
DOI: 10.1021/bm500670r
Rights: © 2014 American Chemical Society.
Fulltext Permission: none
Fulltext Availability: No Fulltext
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