Please use this identifier to cite or link to this item:
https://hdl.handle.net/10356/106440
Title: | Microscopy using randomized speckle illumination | Authors: | Perinchery, Sandeep Menon Shinde, Anant Murukeshan, Vadakke Matham |
Keywords: | Structure Illumination Speckle Engineering::Mechanical engineering |
Issue Date: | 2017 | Source: | Perinchery, S. M., Shinde, A., & Vadakke Matham, M. (2017). Microscopy using randomized speckle illumination. Proceedings of SPIE - Fifth International Conference on Optical and Photonics Engineering, 10449, 104490H-. doi:10.1117/12.2269564 | Series/Report no.: | Proceedings of SPIE - International Conference on Optical and Photonics Engineering | Abstract: | It is well known for structured illumination microscopy (SIM) that the lateral resolution by a factor of two beyond the classical diffraction limit is achieved using spatially structured illumination in wide-field fluorescence microscope. In the state of art SIM systems, grating patterns are generally generated by physical gratings or by spatial light modulators such as digital micro mirrors (DMD), liquid crystal displays (LCD). In this study, using a combination of LCD and ground glasses, size controlled randomized speckle patterns are generated as an illumination source for the microscope. Proof of concept of using speckle illumination in SIM configuration is tested by imaging fixed BPAE cells. | URI: | https://hdl.handle.net/10356/106440 http://hdl.handle.net/10220/49637 |
ISSN: | 0277-786X | DOI: | 10.1117/12.2269564 | Rights: | © 2017 SPIE. All rights reserved. This paper was published in Proceedings of SPIE - Fifth International Conference on Optical and Photonics Engineering and is made available with permission of SPIE. | Fulltext Permission: | open | Fulltext Availability: | With Fulltext |
Appears in Collections: | MAE Journal Articles |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Microscopy using randomized speckle illumination.pdf | 348.91 kB | Adobe PDF | ![]() View/Open |
Page view(s)
222
Updated on Feb 5, 2023
Download(s) 50
72
Updated on Feb 5, 2023
Google ScholarTM
Check
Altmetric
Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.