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Title: A novel non-sequencing approach for rapid authentication of Testudinis Carapax et Plastrum and Trionycis Carapax by species-specific primers
Authors: Yang, Huan
Yu, Pingtian
Lu, Yi
Jiao, Zhaoqun
Chen, Liqun
Zhou, Ying
Shen, Yuping
Jia, Xiaobin
Keywords: Polymerase Chain Reaction
Science::Biological sciences
Animal-derived Chinese Medicine
Issue Date: 2018
Source: Yang, H., Yu, P., Lu, Y., Jiao, Z., Chen, L., Zhou, Y., . . . Jia, X. (2018). A novel non-sequencing approach for rapid authentication of Testudinis Carapax et Plastrum and Trionycis Carapax by species-specific primers. Royal Society Open Science, 5(4), 172140-. doi:10.1098/rsos.172140
Series/Report no.: Royal Society Open Science
Abstract: A novel non-sequencing approach was developed to detect short DNA fragments (ca 100 bp) for rapid authentication of two natural products, namely Testudinis Carapax et Plastrum and Trionycis Carapax, based on the difference in mitochondrial genome. Five specifically designed primer reactions were established to target species for reliable identification of their commercial products. They were confirmed to have a high level of inter-species-specificity and good intra-species stability. The limit of detection was estimated to be 1 ng of genomes for all of five assays. Also, the validation results demonstrated that the raw materials and processed products in addition to some of the highly processed products can be conveniently authenticated with good sensitivity and precision by this newly proposed approach. Especially, when reference sample mixtures were assayed, these primer sets have still performed well but not the prevailing COI barcoding technology. These could assist in the discrimination and identification of other animal-derived medicines for their form of raw material, the pulverized and the complex.
DOI: 10.1098/rsos.172140
Schools: School of Biological Sciences 
Rights: © 2018 The Author(s). Published by the Royal Society under the terms of the Creative Commons Attribution License, which permits unrestricted use, provided the original author and source are credited.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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