Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/106705
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dc.contributor.authorArumugam, Krithikaen
dc.contributor.authorBağcı, Caneren
dc.contributor.authorBessarab, Irinaen
dc.contributor.authorBeier, Sinaen
dc.contributor.authorBuchfink, Benjaminen
dc.contributor.authorGórska, Annaen
dc.contributor.authorQiu, Guangleien
dc.contributor.authorHuson, Daniel H.en
dc.contributor.authorWilliams, Rohan B. H.en
dc.date.accessioned2019-07-01T04:14:48Zen
dc.date.accessioned2019-12-06T22:16:32Z-
dc.date.available2019-07-01T04:14:48Zen
dc.date.available2019-12-06T22:16:32Z-
dc.date.issued2019en
dc.identifier.citationArumugam, K., Bağcı, C., Bessarab, I., Beier, S., Buchfink, B., Górska, A., . . . Williams, R. B. H. (2019). Annotated bacterial chromosomes from frame-shift-corrected long-read metagenomic data. Microbiome, 7(1), 61-. doi:10.1186/s40168-019-0665-yen
dc.identifier.urihttps://hdl.handle.net/10356/106705-
dc.description.abstractBackground : Short-read sequencing technologies have long been the work-horse of microbiome analysis. Continuing technological advances are making the application of long-read sequencing to metagenomic samples increasingly feasible. Results : We demonstrate that whole bacterial chromosomes can be obtained from an enriched community, by application of MinION sequencing to a sample from an EBPR bioreactor, producing 6 Gb of sequence that assembles into multiple closed bacterial chromosomes. We provide a simple pipeline for processing such data, which includes a new approach to correcting erroneous frame-shifts. Conclusions : Advances in long-read sequencing technology and corresponding algorithms will allow the routine extraction of whole chromosomes from environmental samples, providing a more detailed picture of individual members of a microbiome.en
dc.description.sponsorshipNRF (Natl Research Foundation, S’pore)en
dc.description.sponsorshipMOE (Min. of Education, S’pore)en
dc.format.extent13 p.en
dc.language.isoenen
dc.relation.ispartofseriesMicrobiomeen
dc.rights© 2019 The Author(s). This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.en
dc.subjectMicrobiomeen
dc.subjectDRNTU::Engineering::Environmental engineeringen
dc.subjectLong-read Sequencingen
dc.titleAnnotated bacterial chromosomes from frame-shift-corrected long-read metagenomic dataen
dc.typeJournal Articleen
dc.contributor.researchSingapore Centre for Environmental Life Sciences and Engineeringen
dc.identifier.doi10.1186/s40168-019-0665-yen
dc.description.versionPublished versionen
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