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dc.contributor.authorArumugam, Krithikaen
dc.contributor.authorBağcı, Caneren
dc.contributor.authorBessarab, Irinaen
dc.contributor.authorBeier, Sinaen
dc.contributor.authorBuchfink, Benjaminen
dc.contributor.authorGórska, Annaen
dc.contributor.authorQiu, Guangleien
dc.contributor.authorHuson, Daniel H.en
dc.contributor.authorWilliams, Rohan B. H.en
dc.identifier.citationArumugam, K., Bağcı, C., Bessarab, I., Beier, S., Buchfink, B., Górska, A., . . . Williams, R. B. H. (2019). Annotated bacterial chromosomes from frame-shift-corrected long-read metagenomic data. Microbiome, 7(1), 61-. doi:10.1186/s40168-019-0665-yen
dc.description.abstractBackground : Short-read sequencing technologies have long been the work-horse of microbiome analysis. Continuing technological advances are making the application of long-read sequencing to metagenomic samples increasingly feasible. Results : We demonstrate that whole bacterial chromosomes can be obtained from an enriched community, by application of MinION sequencing to a sample from an EBPR bioreactor, producing 6 Gb of sequence that assembles into multiple closed bacterial chromosomes. We provide a simple pipeline for processing such data, which includes a new approach to correcting erroneous frame-shifts. Conclusions : Advances in long-read sequencing technology and corresponding algorithms will allow the routine extraction of whole chromosomes from environmental samples, providing a more detailed picture of individual members of a microbiome.en
dc.description.sponsorshipNRF (Natl Research Foundation, S’pore)en
dc.description.sponsorshipMOE (Min. of Education, S’pore)en
dc.format.extent13 p.en
dc.rights© 2019 The Author(s). This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.en
dc.subjectDRNTU::Engineering::Environmental engineeringen
dc.subjectLong-read Sequencingen
dc.titleAnnotated bacterial chromosomes from frame-shift-corrected long-read metagenomic dataen
dc.typeJournal Articleen
dc.contributor.researchSingapore Centre for Environmental Life Sciences and Engineeringen
dc.description.versionPublished versionen
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