Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/106905
Title: “Turn-on” fluorescence probe integrated polymer nanoparticles for sensing biological thiol molecules
Authors: Li, Menghuan
Li, Peizhou
Zhang, Quan
Selvan, Subramanian Tamil
Zhao, Yanli
Ang, Chung Yen
Tan, Si Yu
Lu, Yunpeng
Bai, Linyi
Keywords: DRNTU::Engineering::Nanotechnology
Issue Date: 2014
Source: Ang, C. Y., Tan, S. Y., Lu, Y., Bai, L., Li, M., Li, P., et al. (2014). “Turn-on” fluorescence probe integrated polymer nanoparticles for sensing biological thiol molecules. Scientific reports, 4.
Series/Report no.: Scientific reports
Abstract: A “turn-on” thiol-responsive fluorescence probe was synthesized and integrated into polymeric nanoparticles for sensing intracellular thiols. There is a photo-induced electron transfer process in the off state of the probe, and this process is terminated upon the reaction with thiol compounds. Configuration interaction singles (CIS) calculation was performed to confirm the mechanism of this process. A series of sensing studies were carried out, showing that the probe-integrated nanoparticles were highly selective towards biological thiol compounds over non-thiolated amino acids. Kinetic studies were also performed to investigate the relative reaction rate between the probe and the thiolated amino acids. Subsequently, the Gibbs free energy of the reactions was explored by means of the electrochemical method. Finally, the detection system was employed for sensing intracellular thiols in cancer cells, and the sensing selectivity could be further enhanced with the use of a cancer cell-targeting ligand in the nanoparticles. This development paves a path for the sensing and detection of biological thiols, serving as a potential diagnostic tool in the future.
URI: https://hdl.handle.net/10356/106905
http://hdl.handle.net/10220/25237
ISSN: 2045-2322
DOI: 10.1038/srep07057
Rights: This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/
Fulltext Permission: open
Fulltext Availability: With Fulltext
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