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|Title:||Nitrile reductase as a biocatalyst : opportunities and challenges||Authors:||Yang, Lifeng
Koh, Siew Lee
Sutton, Peter W.
|Keywords:||DRNTU::Science::Biological sciences||Issue Date:||2014||Source:||Yang, L., Koh, S. L., Sutton, P. W., & Liang, Z.-X. (2014). Nitrile reductase as a biocatalyst : opportunities and challenges. Catalysis science & technology, 4(9), 2871-2876.||Series/Report no.:||Catalysis science & technology||Abstract:||Nitrile-containing compounds are widely manufactured and extensively used in the chemical and pharmaceutical industries as synthetic intermediates or precursors. Nitrile hydratase and nitrilase have been successfully developed as biocatalysts for the production of amides and carboxylic acids from nitrile precursors. The discovery of a family of nitrile reductases that catalyse the reduction of nitrile to amine raised the hope of developing environmentally sustainable nitrile-reducing biocatalysts to replace metal hydride catalysts. However, ten years after the discovery of the QueF nitrile reductases, little progress has been made towards the development of nitrile reductase biocatalysts with altered or broadened substrate specificity. In this article, we analyse and review the structure and catalytic mechanism of QueF nitrile reductases and other structurally related T-fold family enzymes. We argue that the poor evolvability of the T-fold enzymes and the kinetically sluggish reaction catalysed by QueFs pose formidable challenges for developing this family of enzymes into practically useful biocatalysts. The challenges do not seem to be mitigated by current computational design or directed-evolution methods. Searching for another family of nitrile reductases or engineering a more evolvable protein scaffold to support the nitrile-reducing chemistry may be a more viable strategy to develop a nitrile reductase biocatalyst despite another set of foreseeable challenges.||URI:||https://hdl.handle.net/10356/106913
|DOI:||10.1039/C4CY00646A||Rights:||© 2014 The Royal Society of Chemistry. This is the author created version of a work that has been peer reviewed and accepted for publication by Catalysis Science & Technology, The Royal Society of Chemistry. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1039/C4CY00646A].||Fulltext Permission:||open||Fulltext Availability:||With Fulltext|
|Appears in Collections:||SBS Journal Articles|
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