Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/106920
Title: A microfluidic gradient mixer-flow chamber as a new tool to study biofilm development under defined solute gradients
Authors: Zhang, Yingdan
Li, Cheng
Wu, Yichao
Zhang, Yilei
Zhou, Zhi
Cao, Bin
Keywords: Biofilm
Solute Gradient
Engineering::Civil engineering
Issue Date: 2019
Source: Zhang, Y., Li, C., Wu, Y., Zhang, Y., Zhou, Z., & Cao, B. (2019). A microfluidic gradient mixer-flow chamber as a new tool to study biofilm development under defined solute gradients. Biotechnology and Bioengineering, 116(1), 54-64. doi:10.1002/bit.26852
Series/Report no.: Biotechnology and Bioengineering
Abstract: Understanding the dynamics of biofilm development in response to chemical cues and signals is required toward the development of controllable biofilm‐mediated bioprocesses. In this study, we report a new biofilm growth system that integrates a microfluidic gradient mixer with a biofilm growth chamber. The biofilm growth system allows biofilms to grow under defined solute gradients and enables nondestructive monitoring of the biofilm development dynamics in response to the defined gradients. The solute gradients generated in the system were simulated and then validated experimentally. We then demonstrated the applicability of the biofilm growth system in studying biofilm development under defined solute gradients. Specifically, we examined biofilm development of Shewanella oneidensis and Comamonas testosteroni under a defined calcium and nitrate gradient, respectively. Using two C. testosteroni strains (WDL7 and I2), we further demonstrated the applicability of our biofilm growth system to study the development of coculture biofilms under a defined solute gradient. Our results show that the biofilm growth system we have developed here can be a promising tool to reveal the dynamics of biofilm development in response to chemical cues and signals as well as the interorganism interactions in coculture biofilms.
URI: https://hdl.handle.net/10356/106920
http://hdl.handle.net/10220/50252
ISSN: 0006-3592
DOI: 10.1002/bit.26852
Rights: This is the peer reviewed version of the following article: Zhang, Y., Li, C., Wu, Y., Zhang, Y., Zhou, Z., & Cao, B. (2019). A microfluidic gradient mixer-flow chamber as a new tool to study biofilm development under defined solute gradients. Biotechnology and Bioengineering, 116(1), 54-64., which has been published in final form at http://dx.doi.org/10.1002/bit.26852. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Fulltext Permission: open
Fulltext Availability: With Fulltext
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