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Title: In-fiber photo-immobilization of a bioactive surface
Authors: Lee, Elizabeth
Yong, Derrick
Yu, Xia
Li, Hao
Chan, Chi Chiu
Keywords: DRNTU::Science::Medicine::Biomedical engineering
Issue Date: 2014
Source: Lee, E., Yong, D., Yu, X., Li, H., & Chan, C. C. (2014). In-fiber photo-immobilization of a bioactive surface. Journal of biomedical optics, 19(12).
Series/Report no.: Journal of biomedical optics
Abstract: We demonstrate the first in-fiber light-induced bioactive biotin-functionalization via photobleaching fluorophore-conjugated biotin. Photobleaching the fluorophores generated free radicals that bind to the albumin-passivated inner surface of pure silica photonic crystal fiber. The subsequent attachment of dye-conjugated streptavidin to the bound biotin qualified the photo-immobilization process and demonstrated a potential for the construction of in-fiber macromolecular assemblies or multiplexes. Compared with other in-fiber bioactive coating methods, the proposed light-induced technique requires only a low-power light source, without the need for additional preactivation steps or toxic chemical reagents. This method, hence, enables a simple and compact implementation for potential biomedical applications.
ISSN: 1083-3668
DOI: 10.1117/1.JBO.19.12.120502
Rights: © 2014 Society of Photo-optical Instrumentation Engineers. This paper was published in Journal of Biomedical Optics and is made available as an electronic reprint (preprint) with permission of Society of Photo-optical Instrumentation Engineers. The paper can be found at the following official DOI: []. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.
Fulltext Permission: open
Fulltext Availability: With Fulltext
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