Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/107080
Title: Bioconversion of styrene to poly(hydroxyalkanoate) (PHA) by a new bacterial strain Pseudomonas putida NBUS12
Authors: Tan, Giin-Yu Amy
Chen, Chia-Lung
Ge, Liya
Li, Ling
Tan, Swee Ngin
Wang, Jing-Yuan
Keywords: DRNTU::Science::Biological sciences::Microbiology::Bacteria
Issue Date: 2015
Source: Tan, G.-Y. A., Chen, C.-L., Ge, L., Li, L., Tan, S. N., & Wang, J.-Y. (2015). Bioconversion of styrene to poly(hydroxyalkanoate) (PHA) by a new bacterial strain Pseudomonas putida NBUS12
Series/Report no.: Microbes and environments
Abstract: Styrene is a toxic pollutant commonly found in waste effluents from plastic processing industries. We herein identified and characterized microorganisms for bioconversion of the organic eco-pollutant styrene into a valuable biopolymer medium-chain-length poly(hydroxyalkanoate) (mcl-PHA). Twelve newly-isolated styrene-degrading Pseudomonads were obtained and partial phaC genes were detected by PCR in these isolates. These isolates assimilated styrene to produce mcl-PHA, forming PHA contents between 0.05±0.00 and 23.10±3.25% cell dry mass (% CDM). The best-performing isolate was identified as Pseudomonas putida NBUS12. A genetic analysis of 16S rDNA and phaZ genes revealed P. putida NBUS12 as a genetically-distinct strain from existing phenotypically-similar bacterial strains. This bacterium achieved a final biomass of 1.28±0.10 g L−1 and PHA content of 32.49±2.40% CDM. The extracted polymer was mainly comprised of 3-hydroxyhexanoate (C6 ), 3-hydroxyoctanoate (C8 ), 3-hydroxydecanoate (C10 ), 3-hydroxydodecanoate (C12 ), and 3-hydroxytetradecanoate (C14 ) monomers at a ratio of 2:42:1257:17:1. These results collectively suggested that P. putida NBUS12 is a promising candidate for the biotechnological conversion of styrene into mcl-PHA.
URI: https://hdl.handle.net/10356/107080
http://hdl.handle.net/10220/25587
DOI: 10.1264/jsme2.ME14138
Rights: © 2015 Japanese Society of Microbial Ecology. This paper was published in Microbes and Environments and is made available as an electronic reprint (preprint) with permission of Japanese Society of Microbial Ecology. The paper can be found at the following official DOI: [http://dx.doi.org/10.1264/jsme2.ME14138]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.
Fulltext Permission: open
Fulltext Availability: With Fulltext
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