Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/107327
Title: Effects of permeability and living space on cell fate and neo-tissue development in hydrogel-based scaffolds : a study with cartilaginous model
Authors: Fan, Changjiang
Wang, Dong-An
Keywords: DRNTU::Science::Biological sciences
Issue Date: 2015
Source: Fan, C., & Wang, D.-A. (2015). Effects of permeability and living space on cell fate and neo-tissue development in hydrogel-based scaffolds : a study with cartilaginous model. Macromolecular bioscience, 15(4), 535-545.
Series/Report no.: Macromolecular bioscience
Abstract: One bottleneck in tissue regeneration with hydrogel scaffolds is the limited understanding of the crucial factors for controlling hydrogel's physical microenvironments to regulate cell fate. Here, the effects of permeability and living space of hydrogels on encapsulated cells' behavior were evaluated, respectively. Three model hydrogel-based constructs are fabricated by using photo-crosslinkable hyaluronic acid as precursor and chondrocytes as model cell type. The better permeable hydrogels facilitate better cell viability and rapid proliferation, which lead to increased production of extracellular matrix (ECM), e.g. collagen, glycosaminoglycan. By prolonged culture, nano-sized hydrogel networks inhibit neo-tissue development, and the presence of macro-porous living spaces significantly enhance ECM deposition via forming larger cell clusters and eventually induce formation of scaffold-free neo-tissue islets. The results of this work demonstrate that the manipulation and optimization of hydrogel microenvironments, namely permeability and living space, are crucial to direct cell fate and neo-tissue formation.
URI: https://hdl.handle.net/10356/107327
http://hdl.handle.net/10220/25541
ISSN: 1616-5187
DOI: 10.1002/mabi.201400453
Rights: © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:SCBE Journal Articles

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