Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/12889
Full metadata record
DC FieldValueLanguage
dc.contributor.authorChen, Weien
dc.date.accessioned2008-10-15T03:23:35Zen
dc.date.available2008-10-15T03:23:35Zen
dc.date.copyright2008en
dc.date.issued2008en
dc.identifier.citationChen, W. (2008). Polypyrrole and its nanocomposite based electrochemical immunosensors. Doctoral thesis, Nanyang Technological University, Singapore.en
dc.identifier.urihttps://hdl.handle.net/10356/12889en
dc.description.abstractThe goal of this research is studies of polypyrrole (PPy), polypyrrole propylic acid (PPa) and PPy nanocomposite based electrochemical immunosensors for high sensitivity and good specificity. The nanocomposite was electrochemically one-step synthesized to incorporate gold nanoparticles and PPa into PPy matrix for significantly improving the stability and electroactivity of the film. In situ atomic force microscopy (AFM) associated with simultaneously electrochemical measurements was employed to study the nucleation and growth process and a mechanism is proposed accordingly. An impedimetric immunosensor based on the nanocomposite was designed and fabricated, and a novel data processing method was investigated. Normalized impedance was introduced to present the unit impedance change for reducing the variation of the bulk electronic properties of Ab/PPy films and then improving the signal to noise ratio (S/N). PPa as a component in the sensing composite film provides high porosity and hydrophilicity for sensitivity enhancement, while introducing high density of carboxyl groups of PPa for covalent immobilization of protein probes, leading to significantly improved detection sensitivity compared with conventional entrapment methods. As a model, anti-mouse IgG was covalently immobilized or entrapped in the PPa film and used in a sandwich-type alkaline phosphatase-catalyzing amperometric immunoassay with p-aminophenyl phosphate as the substrate, demonstrating remarkable improvement of the immunoassay sensitivity.en
dc.format.extent181 p.en
dc.language.isoenen
dc.subjectDRNTU::Science::Medicine::Biosensorsen
dc.subjectDRNTU::Science::Chemistry::Organic chemistry::Heterocyclic compoundsen
dc.titlePolypyrrole and its nanocomposite based electrochemical immunosensorsen
dc.typeThesisen
dc.contributor.supervisorLi Changmingen
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen
dc.description.degreeDOCTOR OF PHILOSOPHY (SCBE)en
dc.identifier.doi10.32657/10356/12889en
item.grantfulltextopen-
item.fulltextWith Fulltext-
Appears in Collections:SCBE Theses
Files in This Item:
File Description SizeFormat 
ChenWei08.pdfMain report2.74 MBAdobe PDFThumbnail
View/Open

Page view(s) 10

586
Updated on Sep 18, 2021

Download(s) 20

211
Updated on Sep 18, 2021

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.