Please use this identifier to cite or link to this item:
Title: Design, synthesis, and application of electroactive reporters for nucleic acid biosensing
Authors: Tansil, Natalia Chendrawati
Keywords: DRNTU::Engineering::Materials::Biomaterials
DRNTU::Science::Chemistry::Analytical chemistry::Nucleic acids
Issue Date: 2008
Source: Tansil, N. C. (2008). Design, synthesis, and application of electroactive reporters for nucleic acid biosensing. Doctoral thesis, Nanyang Technological University, Singapore.
Abstract: This thesis discusses the design, synthesis, and application of electroactive reporters for nucleic acid biosensing. The reporters are able to specifically recognize and sensitively report the hybridization of target nucleic acids to capture probes in an electrochemical biosensor. Three compounds were used in different detection schemes. In each scheme, direct detection was achieved by measuring the electroactivity of Os or Ru complex. Lower detection limits were obtained through various amplification strategies. The Os complex catalytically oxidizes ascorbic acid, leading to an amplified current signal in amperometry and a detection limit of 60 fM for oligonucleotide samples. The Ru complex catalytically oxidizes guanine bases, leading to an increase of peak current in cyclic voltammetry and a detection limit of 1.5 pM for TP53 cDNA. The EDOT moiety acts as ‘seeds’ that facilitates selective polymerization of poly(EDOT). Under controlled conditions, the amount of polymer deposited was positively related to target DNA concentrations as low as 20 pM. The advantageous electrochemical and binding properties of the newly synthesized reporters qualify them for application in electrochemical detection platforms that can potentially be incorporated into point-of-care nucleic acid biosensors.
DOI: 10.32657/10356/13541
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MSE Theses

Files in This Item:
File Description SizeFormat 
Tmse0302302G.pdf4.52 MBAdobe PDFThumbnail

Page view(s) 50

Updated on May 11, 2021

Download(s) 5

Updated on May 11, 2021

Google ScholarTM




Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.