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|Title:||The genetic and epigenetic architecture of sexual dimorphism in tilapia||Authors:||Wan, Zi Yi||Keywords:||Science::Biological sciences::Genetics||Issue Date:||2019||Publisher:||Nanyang Technological University||Source:||Wan, Z. Y. (2019). The genetic and epigenetic architecture of sexual dimorphism in tilapia. Doctoral thesis, Nanyang Technological University, Singapore.||Abstract:||Tilapia is an important aquaculture species. Males outgrow females. I explored the genetic and epigenetic architecture of male and female tilapia to understand more about sexual dimorphism. Whole genome bisulfite sequencing and RNA-seq in skeletal muscle revealed sexually-dimorphic methylated regions and sex-biased gene expression. dN/dS analysis uncovered that sex-biased genes in tilapia somatic tissues were under relaxed purifying selection. Analysis of brain transcriptomes identified 124, 55 and 2706 sex-biased genes at 5, 30 and 90 days post hatch, respectively. The pro-opiomelanocortin (pomc) gene, which was female-biased in the brain, was selected for functional analysis. pomc knock-out zebrafish showed faster growth and higher sensitivity to feeding compared to wildtype. Two estrogenic response elements upstream of tilapia pomc were sensitive to estrogen induction in a luciferase reporter assay. These results suggest that pomc may be involved in sexual size dimorphism. In addition, I identified SNPs located upstream of rasgrf1, which were associated with increased growth rate and may be useful in selecting fast-growing tilapia.||URI:||https://hdl.handle.net/10356/136866||DOI:||10.32657/10356/136866||Rights:||This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).||Fulltext Permission:||embargo_20220118||Fulltext Availability:||With Fulltext|
|Appears in Collections:||SBS Theses|
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