Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/136866
Title: The genetic and epigenetic architecture of sexual dimorphism in tilapia
Authors: Wan, Zi Yi
Keywords: Science::Biological sciences::Genetics
Issue Date: 2019
Publisher: Nanyang Technological University
Source: Wan, Z. Y. (2019). The genetic and epigenetic architecture of sexual dimorphism in tilapia. Doctoral thesis, Nanyang Technological University, Singapore.
Abstract: Tilapia is an important aquaculture species. Males outgrow females. I explored the genetic and epigenetic architecture of male and female tilapia to understand more about sexual dimorphism. Whole genome bisulfite sequencing and RNA-seq in skeletal muscle revealed sexually-dimorphic methylated regions and sex-biased gene expression. dN/dS analysis uncovered that sex-biased genes in tilapia somatic tissues were under relaxed purifying selection. Analysis of brain transcriptomes identified 124, 55 and 2706 sex-biased genes at 5, 30 and 90 days post hatch, respectively. The pro-opiomelanocortin (pomc) gene, which was female-biased in the brain, was selected for functional analysis. pomc knock-out zebrafish showed faster growth and higher sensitivity to feeding compared to wildtype. Two estrogenic response elements upstream of tilapia pomc were sensitive to estrogen induction in a luciferase reporter assay. These results suggest that pomc may be involved in sexual size dimorphism. In addition, I identified SNPs located upstream of rasgrf1, which were associated with increased growth rate and may be useful in selecting fast-growing tilapia.
URI: https://hdl.handle.net/10356/136866
DOI: 10.32657/10356/136866
Rights: This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).
Fulltext Permission: embargo_20220118
Fulltext Availability: With Fulltext
Appears in Collections:SBS Theses

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