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dc.contributor.authorKim, Purumen_US
dc.identifier.citationKim, P. (2019). Understanding the biological functions of POPX2 phosphatase through its interactome. Doctoral thesis, Nanyang Technological University, Singapore.en_US
dc.description.abstractPOPX2 (Partner of PIX 2) is a serine/threonine phosphatase known to dephosphorylate PAK1, CaMKII and TAK1. POPX2 has been reported to be positively associated with cell motility and invasiveness of breast cancer cells. In order to further investigate the roles and functions of POPX2 in the cells, we have adopted two approaches to identify POPX2-interacting proteins. In the first approach, we pulled down Flag-tagged POPX2 and determined the co-precipitated proteins using mass spectrometry (MS). We discovered Coronin 1C (Coro 1C) as a potential POPX2-interacting protein. In the second approach, we used a combination of SILAC-MS proteomics and bioinformatic analysis and identified Check point kinase 1 (Chk1) as a binding partner and possible substrate of POPX2.en_US
dc.publisherNanyang Technological Universityen_US
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).en_US
dc.subjectScience::Biological sciences::Molecular biologyen_US
dc.titleUnderstanding the biological functions of POPX2 phosphatase through its interactomeen_US
dc.typeThesis-Doctor of Philosophyen_US
dc.contributor.supervisorKoh Cheng Geeen_US
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.description.degreeDoctor of Philosophyen_US
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