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Title: Understanding the biological functions of POPX2 phosphatase through its interactome
Authors: Kim, Purum
Keywords: Science::Biological sciences::Molecular biology
Issue Date: 2019
Publisher: Nanyang Technological University
Source: Kim, P. (2019). Understanding the biological functions of POPX2 phosphatase through its interactome. Doctoral thesis, Nanyang Technological University, Singapore.
Abstract: POPX2 (Partner of PIX 2) is a serine/threonine phosphatase known to dephosphorylate PAK1, CaMKII and TAK1. POPX2 has been reported to be positively associated with cell motility and invasiveness of breast cancer cells. In order to further investigate the roles and functions of POPX2 in the cells, we have adopted two approaches to identify POPX2-interacting proteins. In the first approach, we pulled down Flag-tagged POPX2 and determined the co-precipitated proteins using mass spectrometry (MS). We discovered Coronin 1C (Coro 1C) as a potential POPX2-interacting protein. In the second approach, we used a combination of SILAC-MS proteomics and bioinformatic analysis and identified Check point kinase 1 (Chk1) as a binding partner and possible substrate of POPX2.
DOI: 10.32657/10356/136952
Rights: This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Theses

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