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Title: Vascular bed molecular profiling by differential systemic decellularization in vivo
Authors: Serra, Aida
Gallart-Palau, Xavier
Park, Jung Eun
Lim, Grace Gui Yin
Lim, Kah Leong
Ho, Hee Hwa
Tam, James Pingkwan
Sze, Siu Kwan
Keywords: Science::Biological sciences
Issue Date: 2018
Source: Serra, A., Gallart-Palau, X., Park, J. E., Lim, G. G. Y., Lim, K. L., Ho, H. H., . . . Sze, S. K. (2018). Vascular bed molecular profiling by differential systemic decellularization in vivo, Arteriosclerosis, Thrombosis, and Vascular Biology, 38, 10, 2396-2409. doi:10.1161/ATVBAHA.118.311552
Journal: Arteriosclerosis, Thrombosis, and Vascular Biology
Abstract: Objective- Vascular endothelial dysfunction is a key component of several major human diseases, but the molecular basis of this complex disorder has been difficult to determine in vivo. Previous attempts to identify key mediators of vascular endothelial dysfunction in experimental models have been limited by the lack of suitable methods for system-wide analyses of vascular bed biology. Here, we aimed to develop a novel method for investigating vascular endothelial dysfunction pathogenesis that enables system-wide analyses of molecular interactions between endothelial glycocalyx, endothelial cells, and smooth muscle cells in murine. Approach and Results- We developed a new technique using whole-body differential perfusion with increasing concentrations of detergent buffer to selectively solubilize distinct layers of vascular bed tissue in rodents. When combined with proteomics techniques, our novel approach of differential systemic decellularization in vivo enabled quantitative profiling of vascular beds throughout the body. Initial perfusion with phosphate buffer was used to obtain the endothelial glycocalyx, followed by subsequent extraction of endothelial cell components, and finally by smooth muscle cell constituents with increasing concentrations of detergent. Differential systemic decellularization in vivo has also been successfully applied to characterize molecular events in the vascular bed pathology of lipopolysaccharide-challenged mice. Conclusions- Together, these data indicate that differential systemic decellularization in vivo permits system-wide molecular characterization of vascular bed proteomes in rodent models and can be used to advance our current understanding of vascular endothelial dysfunction pathogenesis and progression in a wide range of disease settings.
ISSN: 1079-5642
DOI: 10.1161/ATVBAHA.118.311552
Schools: School of Biological Sciences 
Rights: © 2018 American Heart Association, Inc. All rights reserved. This paper was published in Arteriosclerosis, Thrombosis, and Vascular Biology and is made available with permission of American Heart Association, Inc.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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