Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/137169
Title: Circulating tumor cell phenotyping via high‐throughput acoustic separation
Authors: Wu, Mengxi
Huang, Po-Hsun
Zhang, Rui
Mao, Zhangming
Chen, Chuyi
Kemeny, Gabor
Li, Peng
Lee, Adrian V.
Gyanchandani, Rekha
Armstrong, Andrew J.
Dao, Ming
Suresh, Subra
Huang, Tony Jun
Keywords: Engineering::Materials
Issue Date: 2018
Source: Wu, M., Huang, P.-H., Zhang, R., Mao, Z., Chen, C., Kemeny, G., . . . Huang, T. J. (2018). Circulating tumor cell phenotyping via high‐throughput acoustic separation. Small, 14(32), 1801131-. doi:10.1002/smll.201801131
Journal: Small
Abstract: The study of circulating tumor cells (CTCs) offers pathways to develop new diagnostic and prognostic biomarkers that benefit cancer treatments. In order to fully exploit and interpret the information provided by CTCs, the development of a platform is reported that integrates acoustics and microfluidics to isolate rare CTCs from peripheral blood in high throughput while preserving their structural, biological, and functional integrity. Cancer cells are first isolated from leukocytes with a throughput of 7.5 mL h-1 , achieving a recovery rate of at least 86% while maintaining the cells' ability to proliferate. High-throughput acoustic separation enables statistical analysis of isolated CTCs from prostate cancer patients to be performed to determine their size distribution and phenotypic heterogeneity for a range of biomarkers, including the visualization of CTCs with a loss of expression for the prostate specific membrane antigen. The method also enables the isolation of even rarer, but clinically important, CTC clusters.
URI: https://hdl.handle.net/10356/137169
ISSN: 1613-6810
DOI: 10.1002/smll.201801131
Rights: This is the peer reviewed version of the following article: Wu, M., Huang, P.-H., Zhang, R., Mao, Z., Chen, C., Kemeny, G., . . . Huang, T. J. (2018). Circulating tumor cell phenotyping via high‐throughput acoustic separation. Small, 14(32), 1801131-. doi:10.1002/smll.201801131, which has been published in final form at https://doi.org/10.1002/smll.201801131. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MSE Journal Articles

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