Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorZhou, Miaomiaoen_US
dc.contributor.authorLee, Bae Hoonen_US
dc.contributor.authorTan, Yu Junen_US
dc.contributor.authorTan, Lay Pohen_US
dc.identifier.citationZhou, M., Lee, B. H., Tan, Y. J., & Tan, L. P. (2019). Microbial transglutaminase induced controlled crosslinking of gelatin methacryloyl to tailor rheological properties for 3D printing. Biofabrication, 11(2), 025011-. doi:10.1088/1758-5090/ab063fen_US
dc.description.abstractGelatin methacryloyl (GelMA) is a versatile biomaterial that has been shown to possess many advantages such as good biocompatibility, support for cell growth, tunable mechanical properties, photocurable capability, and low material cost. Due to these superior properties, much research has been carried out to develop GelMA as a bioink for bioprinting. However, there are still many challenges, and one major challenge is the control of its rheological properties to yield good printability. Herein, this study presents a strategy to control the rheology of GelMA through partial enzymatic crosslinking. Unlike other enzymatic crosslinking strategies where the rheological properties could not be controlled once reaction takes place, we could, to a large extent, keep the rheological properties stable by introducing a deactivation step after obtaining the optimized rheological properties. Ca2+-independent microbial transglutaminase (MTGase) was introduced to partially catalyze covalent bond formation between chains of GelMA. The enzyme was then deactivated to prevent further uncontrolled crosslinking that would render the hydrogel not printable. After printing, a secondary post-printing crosslinking step (photo crosslinking) was then introduced to ensure long-term stability of the printed structure for subsequent cell studies. Biocompatibility studies carried out using cells encapsulated in the printed structure showed excellent cell viability for at least 7 d. This strategy for better control of rheological properties of GelMA could more significantly enhance the usability of this material as bioink for bioprinting of cell-laden structures for soft tissue engineering.en_US
dc.description.sponsorshipNRF (Natl Research Foundation, S’pore)en_US
dc.description.sponsorshipMOE (Min. of Education, S’pore)en_US
dc.rights© 2019 IOP Publishing Ltd. Original content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.en_US
dc.titleMicrobial transglutaminase induced controlled crosslinking of gelatin methacryloyl to tailor rheological properties for 3D printingen_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Materials Science & Engineeringen_US
dc.contributor.schoolSchool of Mechanical and Aerospace Engineeringen_US
dc.contributor.researchSingapore Centre for 3D Printingen_US
dc.description.versionPublished versionen_US
dc.subject.keywordsRheological Propertiesen_US
dc.subject.keywordsGelatin Methacryloylen_US
item.fulltextWith Fulltext-
Appears in Collections:MSE Journal Articles

Citations 5

Updated on Mar 20, 2023

Web of ScienceTM
Citations 5

Updated on Mar 20, 2023

Page view(s)

Updated on Mar 23, 2023

Download(s) 50

Updated on Mar 23, 2023

Google ScholarTM




Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.