Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/137434
Title: An intact IRE1 transmembrane domain is necessary for UPR activation upon lipid bilayer stress in S. cerevisiae
Authors: Ho, Nurulain
Keywords: Science::Biological sciences
Issue Date: 2019
Publisher: Nanyang Technological University
Source: Ho, N. (2019). An intact IRE1 transmembrane domain is necessary for UPR activation upon lipid bilayer stress in S. cerevisiae. Doctoral thesis, Nanyang Technological University, Singapore.
Abstract: Membrane integrity at the endoplasmic reticulum (ER) is compromised in metabolic diseases. Recently, it became evident that such ER lipid aberrations activate the unfolded protein response (UPRLBS). Using a truncated form of the UPR sensor Ire1 that is responsive solely to changes in lipid bilayer composition, we identified pathways, beyond lipid metabolism, required to maintain ER integrity. A lipid sensing switch within the amphipathic helix of Ire1 revealed the importance of the transmembrane domain as a sensor to lipid bilayer stress (LBS). An exciting outcome of our transcriptomic and ChIP-qPCR data confirm novel genes upregulated solely by UPRLBS suggesting an alternative transcriptional programme activated for the adaptation of cells during LBS. This work establishes the broad extent of the UPR, providing insights for future therapeutic interventions.
URI: https://hdl.handle.net/10356/137434
DOI: 10.32657/10356/137434
Rights: This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Theses

Files in This Item:
File Description SizeFormat 
G1502266A_PhD Thesis_Final.pdf6.14 MBAdobe PDFView/Open

Page view(s)

299
Updated on Jan 31, 2023

Download(s) 50

148
Updated on Jan 31, 2023

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.