Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/137714
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dc.contributor.authorSu, Weixinen_US
dc.contributor.authorKumar, Veerendraen_US
dc.contributor.authorDing, Yichenen_US
dc.contributor.authorEro, Ryaen_US
dc.contributor.authorSerra, Aidaen_US
dc.contributor.authorLee, Benjamin Sian Tecken_US
dc.contributor.authorWong, Andrew See Wengen_US
dc.contributor.authorShi, Jianen_US
dc.contributor.authorSze, Siu Kwanen_US
dc.contributor.authorYang, Liangen_US
dc.contributor.authorGao, Yong-Guien_US
dc.date.accessioned2020-04-09T06:33:15Z-
dc.date.available2020-04-09T06:33:15Z-
dc.date.issued2018-
dc.identifier.citationSu, W., Kumar, V., Ding, Y., Ero, R., Serra, A., Lee, B. S. T., . . ., Gao, Y.-G. (2018). Ribosome protection by antibiotic resistance ATP-binding cassette protein. Proceedings of the National Academy of Sciences of the United States of America, 115(20), 5157-5162. doi:10.1073/pnas.1803313115en_US
dc.identifier.issn0027-8424en_US
dc.identifier.urihttps://hdl.handle.net/10356/137714-
dc.description.abstractThe ribosome is one of the richest targets for antibiotics. Unfortunately, antibiotic resistance is an urgent issue in clinical practice. Several ATP-binding cassette family proteins confer resistance to ribosome-targeting antibiotics through a yet unknown mechanism. Among them, MsrE has been implicated in macrolide resistance. Here, we report the cryo-EM structure of ATP form MsrE bound to the ribosome. Unlike previously characterized ribosomal protection proteins, MsrE is shown to bind to ribosomal exit site. Our structure reveals that the domain linker forms a unique needle-like arrangement with two crossed helices connected by an extended loop projecting into the peptidyl-transferase center and the nascent peptide exit tunnel, where numerous antibiotics bind. In combination with biochemical assays, our structure provides insight into how MsrE binding leads to conformational changes, which results in the release of the drug. This mechanism appears to be universal for the ABC-F type ribosome protection proteins.en_US
dc.description.sponsorshipNRF (Natl Research Foundation, S’pore)en_US
dc.description.sponsorshipMOE (Min. of Education, S’pore)en_US
dc.language.isoenen_US
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of Americaen_US
dc.rights© 2018 The Author(s) (Published by National Academy of Sciences). All rights reserved.en_US
dc.subjectScience::Biological sciencesen_US
dc.titleRibosome protection by antibiotic resistance ATP-binding cassette proteinen_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.contributor.organizationInstitute of Structural Biologyen_US
dc.contributor.researchSingapore Centre for Environmental Life Sciences and Engineeringen_US
dc.identifier.doi10.1073/pnas.1803313115-
dc.identifier.pmid29712846-
dc.identifier.scopus2-s2.0-85046954123-
dc.identifier.issue20en_US
dc.identifier.volume115en_US
dc.identifier.spage5157en_US
dc.identifier.epage5162en_US
dc.subject.keywordsRibosome Protectionen_US
dc.subject.keywordsAntibiotic Resistanceen_US
item.fulltextNo Fulltext-
item.grantfulltextnone-
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