Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/137786
Title: Fluorescence-based immunosensor using three-dimensional CNT network structure for sensitive and reproducible detection of oral squamous cell carcinoma biomarker
Authors: Song, Chung Kil
Oh, Eunkyul
Kang, Min Sung
Shin, Bum Seok
Han, Soo Young
Jung, Mingi
Lee, Eui Seok
Yoon, Soo-Young
Sung, Myung Mo
Ng, Wei Beng
Cho, Nam-Joon
Lee, Haiwon
Keywords: Engineering::Materials
Issue Date: 2018
Source: Song, C. K., Oh, E., Kang, M. S., Shin, B. S., Han, S. Y., Jung, M., . . . Lee, H. (2018). Fluorescence-based immunosensor using three-dimensional CNT network structure for sensitive and reproducible detection of oral squamous cell carcinoma biomarker. Analytica Chimica Acta, 1027, 101-108. doi:10.1016/j.aca.2018.04.025
Journal: Analytica Chimica Acta
Abstract: A hierarchical three-dimensional network of carbon nanotubes on Si pillar substrate (3DN-CNTs) was developed for the accurate detection of oral squamous cell carcinoma (OSCC) in clinical saliva samples. The 3DN-CNTs were uniformly coated with a layer of aluminum oxides to enhance structural stability during biomarker detection. Cytokeratin-19 antigen (Cyfra 21-1) was utilized as a model biomarker of OSCC for fluorescence-based immunosensor using 3DN-CNTs (3DN-CNTs sensor). The 3DN-CNTs sensor enhances the sensitivity of Cyfra 21-1 detection by increasing the density of immobilized antibody through high surface area of 3DN-CNTs and enhancing the accessibility of biomolecules through the ordered pathway of hierarchical structure. The reliable detection limit for sensing of Cyfra 21-1 was estimated as in the level of 0.5 ng/mL and the quantitative estimation of Cyfra 21-1 was analyzed by 4-parameter logistic (4-PL) model for curve-fitting analysis. Clinical applicability of 3DN-CNTs sensor was evaluated through correlation with the commercially available electrochemiluminescence (ECL) detection system in the hospital. The assay results of the two systems for clinical saliva samples showed a good linear correlation. The 3DN-CNTs sensor offers great potential for accurate diagnosis of OSCC using Cyfra 21-1 biomarker in clinical fluids.
URI: https://hdl.handle.net/10356/137786
ISSN: 0003-2670
DOI: 10.1016/j.aca.2018.04.025
Rights: © 2018 Elsevier B.V. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:MSE Journal Articles

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