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dc.contributor.authorSong, Chung Kilen_US
dc.contributor.authorOh, Eunkyulen_US
dc.contributor.authorKang, Min Sungen_US
dc.contributor.authorShin, Bum Seoken_US
dc.contributor.authorHan, Soo Youngen_US
dc.contributor.authorJung, Mingien_US
dc.contributor.authorLee, Eui Seoken_US
dc.contributor.authorYoon, Soo-Youngen_US
dc.contributor.authorSung, Myung Moen_US
dc.contributor.authorNg, Wei Bengen_US
dc.contributor.authorCho, Nam-Joonen_US
dc.contributor.authorLee, Haiwonen_US
dc.identifier.citationSong, C. K., Oh, E., Kang, M. S., Shin, B. S., Han, S. Y., Jung, M., . . . Lee, H. (2018). Fluorescence-based immunosensor using three-dimensional CNT network structure for sensitive and reproducible detection of oral squamous cell carcinoma biomarker. Analytica Chimica Acta, 1027, 101-108. doi:10.1016/j.aca.2018.04.025en_US
dc.description.abstractA hierarchical three-dimensional network of carbon nanotubes on Si pillar substrate (3DN-CNTs) was developed for the accurate detection of oral squamous cell carcinoma (OSCC) in clinical saliva samples. The 3DN-CNTs were uniformly coated with a layer of aluminum oxides to enhance structural stability during biomarker detection. Cytokeratin-19 antigen (Cyfra 21-1) was utilized as a model biomarker of OSCC for fluorescence-based immunosensor using 3DN-CNTs (3DN-CNTs sensor). The 3DN-CNTs sensor enhances the sensitivity of Cyfra 21-1 detection by increasing the density of immobilized antibody through high surface area of 3DN-CNTs and enhancing the accessibility of biomolecules through the ordered pathway of hierarchical structure. The reliable detection limit for sensing of Cyfra 21-1 was estimated as in the level of 0.5 ng/mL and the quantitative estimation of Cyfra 21-1 was analyzed by 4-parameter logistic (4-PL) model for curve-fitting analysis. Clinical applicability of 3DN-CNTs sensor was evaluated through correlation with the commercially available electrochemiluminescence (ECL) detection system in the hospital. The assay results of the two systems for clinical saliva samples showed a good linear correlation. The 3DN-CNTs sensor offers great potential for accurate diagnosis of OSCC using Cyfra 21-1 biomarker in clinical fluids.en_US
dc.description.sponsorshipNRF (Natl Research Foundation, S’pore)en_US
dc.description.sponsorshipMOE (Min. of Education, S’pore)en_US
dc.relation.ispartofAnalytica Chimica Actaen_US
dc.rights© 2018 Elsevier B.V. All rights reserved.en_US
dc.titleFluorescence-based immunosensor using three-dimensional CNT network structure for sensitive and reproducible detection of oral squamous cell carcinoma biomarkeren_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Materials Science & Engineeringen_US
dc.subject.keywordsCyfra 21-2en_US
dc.subject.keywordsFluorescence-based Immunoassayen_US
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