Please use this identifier to cite or link to this item:
Title: Mapping QTL for omega-3 content in hybrid saline tilapia
Authors: Lin, Grace
Wang, Le
Ngoh, Si Te
Ji, Lianghui
Orbán, Laszlo
Yue, Gen Hua
Keywords: Science::Biological sciences
Issue Date: 2018
Source: Lin, G., Wang, L., Ngoh, S. T., Ji, L., Orbán, L., & Yue, G. H. (2018). Mapping QTL for omega-3 content in hybrid saline tilapia. Marine biotechnology, 20(1), 10-19. doi:10.1007/s10126-017-9783-3
Journal: Marine biotechnology
Abstract: Tilapia is one of most important foodfish species. The low omega-3 to omega-6 fatty acid ratio in freshwater tilapia meat is disadvantageous for human health. Increasing omega-3 content is an important task in breeding to increase the nutritional value of tilapia. However, conventional breeding to increase omega-3 content is difficult and slow. To accelerate the increase of omega-3 through marker-assisted selection (MAS), we conducted QTL mapping for fatty acid contents and profiles in a F2 family of saline tilapia generated by crossing red tilapia and Mozambique tilapia. The total omega-3 content in F2 hybrid tilapia was 2.5 ± 1.0 mg/g, higher than that (2.00 mg/g) in freshwater tilapia. Genotyping by sequencing (GBS) technology was used to discover and genotype SNP markers, and microsatellites were also genotyped. We constructed a linkage map with 784 markers (151 microsatellites and 633 SNPs). The linkage map was 2076.7 cM long and consisted of 22 linkage groups. Significant and suggestive QTL for total lipid content were mapped on six linkage groups (LG3, -4, -6, -8, -13, and -15) and explained 5.8–8.3% of the phenotypic variance. QTL for omega-3 fatty acids were located on four LGs (LG11, -18, -19, and -20) and explained 5.0 to 7.5% of the phenotypic variance. Our data suggest that the total lipid and omega-3 fatty acid content were determined by multiple genes in tilapia. The markers flanking the QTL for omega-3 fatty acids can be used in MAS to accelerate the genetic improvements of these traits in salt-tolerant tilapia.
ISSN: 1436-2228
DOI: 10.1007/s10126-017-9783-3
Rights: © 2017 Springer Science+Business Media, LLC, part of Springer Nature. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:SBS Journal Articles

Citations 10

checked on Sep 5, 2020

Citations 50

checked on Oct 18, 2020

Page view(s) 50

checked on Oct 20, 2020

Google ScholarTM




Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.