Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/137874
Title: Differential homeostasis of sessile and pendant epithelium reconstituted in a 3D‐printed “GeminiChip”
Authors: Cai, Pingqiang
Li, Zhuyun
Keneth, Ela Sachyani
Wang, Luying
Wan, Changjin
Jiang, Ying
Hu, Benhui
Wu, Yun-Long
Wang, Shutao
Lim, Chwee Teck
Makeyev, Eugene V.
Magdassi, Shlomo
Chen, Xiaodong
Keywords: Science::Biological sciences
Issue Date: 2019
Source: Cai, P., Li, Z., Keneth, E. S., Wang, L., Wan, C., Jiang, Y., . . . Chen X. (2019). Differential homeostasis of sessile and pendant epithelium reconstituted in a 3D‐printed “GeminiChip”. Advanced Materials, 31(28), 1900514-. doi:10.1002/adma.201900514
Journal: Advanced materials
Abstract: Local mechanical cues can affect crucial fate decisions of living cells. Transepithelial stress has been discussed in the context of epithelial monolayers, but the lack of appropriate experimental systems leads current studies to approximate it simply as an in‐plane stress. To evaluate possible contribution of force vectors acting in other directions, double epithelium in a 3D‐printed “GeminiChip” containing a sessile and a pendant channel is reconstituted. Intriguingly, the sessile epithelia is prone to apoptotic cell extrusion upon crowding, whereas the pendant counterpart favors live cell delamination. Transcriptome analyses show upregulation of RhoA, BMP2, and hypoxia‐signaling genes in the pendant epithelium, consistent with the onset of an epithelial–mesenchymal transition program. HepG2 microtumor spheroids also display differential spreading patterns in the sessile and pendant configuration. Using this multilayered GeminiChip, these results uncover a progressive yet critical role of perpendicular force vectors in collective cell behaviors and point at fundamental importance of these forces in the biology of cancer.
URI: https://hdl.handle.net/10356/137874
ISSN: 0935-9648
DOI: 10.1002/adma.201900514
Rights: This is the peer reviewed version of the following article: Cai, P., Li, Z., Keneth, E. S., Wang, L., Wan, C., Jiang, Y., . . . Chen X. (2019). Differential homeostasis of sessile and pendant epithelium reconstituted in a 3D‐printed “GeminiChip”. Advanced Materials, 31(28), 1900514-. doi:10.1002/adma.201900514, which has been published in final form at https://doi.org/10.1002/adma.201900514. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Fulltext Permission: embargo_20200717
Fulltext Availability: With Fulltext
Appears in Collections:MSE Journal Articles

Files in This Item:
File Description SizeFormat 
Cai_et_al_Advanced_Materials_2019.pdf
  Until 2020-07-17
2.58 MBAdobe PDFUnder embargo until Jul 17, 2020

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.