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|Title:||Differential homeostasis of sessile and pendant epithelium reconstituted in a 3D‐printed “GeminiChip”||Authors:||Cai, Pingqiang
Keneth, Ela Sachyani
Lim, Chwee Teck
Makeyev, Eugene V.
|Keywords:||Science::Biological sciences||Issue Date:||2019||Source:||Cai, P., Li, Z., Keneth, E. S., Wang, L., Wan, C., Jiang, Y., . . . Chen X. (2019). Differential homeostasis of sessile and pendant epithelium reconstituted in a 3D‐printed “GeminiChip”. Advanced Materials, 31(28), 1900514-. doi:10.1002/adma.201900514||Journal:||Advanced materials||Abstract:||Local mechanical cues can affect crucial fate decisions of living cells. Transepithelial stress has been discussed in the context of epithelial monolayers, but the lack of appropriate experimental systems leads current studies to approximate it simply as an in‐plane stress. To evaluate possible contribution of force vectors acting in other directions, double epithelium in a 3D‐printed “GeminiChip” containing a sessile and a pendant channel is reconstituted. Intriguingly, the sessile epithelia is prone to apoptotic cell extrusion upon crowding, whereas the pendant counterpart favors live cell delamination. Transcriptome analyses show upregulation of RhoA, BMP2, and hypoxia‐signaling genes in the pendant epithelium, consistent with the onset of an epithelial–mesenchymal transition program. HepG2 microtumor spheroids also display differential spreading patterns in the sessile and pendant configuration. Using this multilayered GeminiChip, these results uncover a progressive yet critical role of perpendicular force vectors in collective cell behaviors and point at fundamental importance of these forces in the biology of cancer.||URI:||https://hdl.handle.net/10356/137874||ISSN:||0935-9648||DOI:||10.1002/adma.201900514||Rights:||This is the peer reviewed version of the following article: Cai, P., Li, Z., Keneth, E. S., Wang, L., Wan, C., Jiang, Y., . . . Chen X. (2019). Differential homeostasis of sessile and pendant epithelium reconstituted in a 3D‐printed “GeminiChip”. Advanced Materials, 31(28), 1900514-. doi:10.1002/adma.201900514, which has been published in final form at https://doi.org/10.1002/adma.201900514. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.||Fulltext Permission:||embargo_20200717||Fulltext Availability:||With Fulltext|
|Appears in Collections:||MSE Journal Articles|
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