Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/138142
Title: Development of an affimer based lateral flow immunoassay using magnetic beads as optical reporters
Authors: Yeo, Keith Shi Jie
Keywords: Engineering::Materials::Biomaterials
Issue Date: 2020
Publisher: Nanyang Technological University
Abstract: Lateral flow immunoassay are growing as an important diagnostic tool due to its convenience and effectiveness. In this report, we explore the effectiveness of conducting a lateral flow immunoassay utilizing affimers as the main bio-recognition molecule over antibodies with magnetic beads as labels. Affimers are man-made protein that are an alternative to antibodies. Affimers are more stable and are more easily produced as compared to animal-derived antibody protein molecules. The use of affimers conjugated to magnetic beads would allow for a more stable and effective testing method, eliminating most of the antibodies limitations. Flow rate on the strip, magnetic bead volume, concentration, the affinity of the magnetic beads to the analyte on the strip and the optimal analyte concentration to be used were also experimented upon to create optimal conditions for the immunoassay. Characterization techniques such as Dynamic light scattering and Scanning electron microscopy were used in the various experiments to determine the success of the protocol.
URI: https://hdl.handle.net/10356/138142
Schools: School of Materials Science and Engineering 
Fulltext Permission: restricted
Fulltext Availability: With Fulltext
Appears in Collections:MSE Student Reports (FYP/IA/PA/PI)

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