Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/138576
Title: Synthetic biohybrid peptidoglycan oligomers enable pan-bacteria-specific labeling and imaging : in vitro and in vivo
Authors: He Jing-Xi
Hoang, Kim Le Mai
Kho, Shu Hui
Guo, Zhong
Zhong, Wenbin
Thappeta, Kishore Reddy Venkata
Zamudio-Vázquez, Rubi
Hoo, Sin Ni
Xiong, Qirong
Duan, Hongwei
Yang, Liang
Chan-Park, Mary Bee Eng
Liu, Xue-Wei.
Keywords: Science::Chemistry
Issue Date: 2020
Source: He, J.-X., Hoang, K. L. M., Kho, S. H., Guo, Z., Zhong, W., Thappeta, K. R. V., . . . Liu, X.-W. (2020). Synthetic biohybrid peptidoglycan oligomers enable pan-bacteria-specific labeling and imaging : in vitro and in vivo. Chemical Science, 11(12), 3171-3179. doi:10.1039/c9sc06345e
Journal: Chemical Science
Abstract: Peptidoglycan is the core component of the bacterial cell wall, which makes it an attractive target for the development of bacterial targeting agents. Intercepting its enzymatic assembly with synthetic substrates allows for labeling and engineering of live bacterial cells. Over the past two decades, small-molecule-based labeling agents, such as antibiotics, D-amino acids or monosaccharides have been developed for probing biological processes in bacteria. Herein, peptidoglycan oligomers, substrates for transglycosylation, are prepared for the first time using a top-down approach, which starts from chitosan as a cheap feedstock. A high efficiency of labeling has been observed in all bacterial strains tested using micromolar substrates. In contrast, uptake into mammalian cells was barely observable. Additional mechanistic studies support a hypothesis of bacteria-specific metabolic labeling rather than non-specific binding to the bacterial surface. Eventually, its practicality in bacterial targeting capability is demonstrated in resistant strain detection and in vivo infection models.
URI: https://hdl.handle.net/10356/138576
ISSN: 2041-6520
DOI: 10.1039/c9sc06345e
Rights: © 2020 The Author(s) (published by Royal Society of Chemistry) (Open Access). This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SPMS Journal Articles

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