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https://hdl.handle.net/10356/138576
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DC Field | Value | Language |
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dc.contributor.author | He Jing-Xi | en_US |
dc.contributor.author | Hoang, Kim Le Mai | en_US |
dc.contributor.author | Kho, Shu Hui | en_US |
dc.contributor.author | Guo, Zhong | en_US |
dc.contributor.author | Zhong, Wenbin | en_US |
dc.contributor.author | Thappeta, Kishore Reddy Venkata | en_US |
dc.contributor.author | Zamudio-Vázquez, Rubi | en_US |
dc.contributor.author | Hoo, Sin Ni | en_US |
dc.contributor.author | Xiong, Qirong | en_US |
dc.contributor.author | Duan, Hongwei | en_US |
dc.contributor.author | Yang, Liang | en_US |
dc.contributor.author | Chan-Park, Mary Bee Eng | en_US |
dc.contributor.author | Liu, Xue-Wei | en_US |
dc.date.accessioned | 2020-05-08T09:05:48Z | - |
dc.date.available | 2020-05-08T09:05:48Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | He, J.-X., Hoang, K. L. M., Kho, S. H., Guo, Z., Zhong, W., Thappeta, K. R. V., . . . Liu, X.-W. (2020). Synthetic biohybrid peptidoglycan oligomers enable pan-bacteria-specific labeling and imaging : in vitro and in vivo. Chemical Science, 11(12), 3171-3179. doi:10.1039/c9sc06345e | en_US |
dc.identifier.issn | 2041-6520 | en_US |
dc.identifier.uri | https://hdl.handle.net/10356/138576 | - |
dc.description.abstract | Peptidoglycan is the core component of the bacterial cell wall, which makes it an attractive target for the development of bacterial targeting agents. Intercepting its enzymatic assembly with synthetic substrates allows for labeling and engineering of live bacterial cells. Over the past two decades, small-molecule-based labeling agents, such as antibiotics, D-amino acids or monosaccharides have been developed for probing biological processes in bacteria. Herein, peptidoglycan oligomers, substrates for transglycosylation, are prepared for the first time using a top-down approach, which starts from chitosan as a cheap feedstock. A high efficiency of labeling has been observed in all bacterial strains tested using micromolar substrates. In contrast, uptake into mammalian cells was barely observable. Additional mechanistic studies support a hypothesis of bacteria-specific metabolic labeling rather than non-specific binding to the bacterial surface. Eventually, its practicality in bacterial targeting capability is demonstrated in resistant strain detection and in vivo infection models. | en_US |
dc.description.sponsorship | MOE (Min. of Education, S’pore) | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartof | Chemical Science | en_US |
dc.rights | © 2020 The Author(s) (published by Royal Society of Chemistry) (Open Access). This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. | en_US |
dc.subject | Science::Chemistry | en_US |
dc.title | Synthetic biohybrid peptidoglycan oligomers enable pan-bacteria-specific labeling and imaging : in vitro and in vivo | en_US |
dc.type | Journal Article | en |
dc.contributor.school | School of Chemical and Biomedical Engineering | en_US |
dc.contributor.school | School of Physical and Mathematical Sciences | en_US |
dc.contributor.organization | NTU Institute for Health Technologies | en_US |
dc.identifier.doi | 10.1039/c9sc06345e | - |
dc.description.version | Accepted version | en_US |
dc.identifier.scopus | 2-s2.0-85082672981 | - |
dc.identifier.issue | 12 | en_US |
dc.identifier.volume | 11 | en_US |
dc.identifier.spage | 3171 | en_US |
dc.identifier.epage | 3179 | en_US |
item.fulltext | With Fulltext | - |
item.grantfulltext | open | - |
Appears in Collections: | SPMS Journal Articles |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
CS manuscript 290120 revised without tracking.pdf | 1.16 MB | Adobe PDF | View/Open | |
CS Supporting information.pdf | 3.12 MB | Adobe PDF | View/Open |
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