Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/138576
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dc.contributor.authorHe Jing-Xien_US
dc.contributor.authorHoang, Kim Le Maien_US
dc.contributor.authorKho, Shu Huien_US
dc.contributor.authorGuo, Zhongen_US
dc.contributor.authorZhong, Wenbinen_US
dc.contributor.authorThappeta, Kishore Reddy Venkataen_US
dc.contributor.authorZamudio-Vázquez, Rubien_US
dc.contributor.authorHoo, Sin Nien_US
dc.contributor.authorXiong, Qirongen_US
dc.contributor.authorDuan, Hongweien_US
dc.contributor.authorYang, Liangen_US
dc.contributor.authorChan-Park, Mary Bee Engen_US
dc.contributor.authorLiu, Xue-Weien_US
dc.date.accessioned2020-05-08T09:05:48Z-
dc.date.available2020-05-08T09:05:48Z-
dc.date.issued2020-
dc.identifier.citationHe, J.-X., Hoang, K. L. M., Kho, S. H., Guo, Z., Zhong, W., Thappeta, K. R. V., . . . Liu, X.-W. (2020). Synthetic biohybrid peptidoglycan oligomers enable pan-bacteria-specific labeling and imaging : in vitro and in vivo. Chemical Science, 11(12), 3171-3179. doi:10.1039/c9sc06345een_US
dc.identifier.issn2041-6520en_US
dc.identifier.urihttps://hdl.handle.net/10356/138576-
dc.description.abstractPeptidoglycan is the core component of the bacterial cell wall, which makes it an attractive target for the development of bacterial targeting agents. Intercepting its enzymatic assembly with synthetic substrates allows for labeling and engineering of live bacterial cells. Over the past two decades, small-molecule-based labeling agents, such as antibiotics, D-amino acids or monosaccharides have been developed for probing biological processes in bacteria. Herein, peptidoglycan oligomers, substrates for transglycosylation, are prepared for the first time using a top-down approach, which starts from chitosan as a cheap feedstock. A high efficiency of labeling has been observed in all bacterial strains tested using micromolar substrates. In contrast, uptake into mammalian cells was barely observable. Additional mechanistic studies support a hypothesis of bacteria-specific metabolic labeling rather than non-specific binding to the bacterial surface. Eventually, its practicality in bacterial targeting capability is demonstrated in resistant strain detection and in vivo infection models.en_US
dc.description.sponsorshipMOE (Min. of Education, S’pore)en_US
dc.language.isoenen_US
dc.relation.ispartofChemical Scienceen_US
dc.rights© 2020 The Author(s) (published by Royal Society of Chemistry) (Open Access). This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence.en_US
dc.subjectScience::Chemistryen_US
dc.titleSynthetic biohybrid peptidoglycan oligomers enable pan-bacteria-specific labeling and imaging : in vitro and in vivoen_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen_US
dc.contributor.schoolSchool of Physical and Mathematical Sciencesen_US
dc.contributor.organizationNTU Institute for Health Technologiesen_US
dc.identifier.doi10.1039/c9sc06345e-
dc.description.versionAccepted versionen_US
dc.identifier.scopus2-s2.0-85082672981-
dc.identifier.issue12en_US
dc.identifier.volume11en_US
dc.identifier.spage3171en_US
dc.identifier.epage3179en_US
item.fulltextWith Fulltext-
item.grantfulltextopen-
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