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Title: A short peptide hydrogel with high stiffness induced by 310-helices to beta-sheet transition in water
Authors: Hiew, Shu Hui
Mohanram, Harini
Ling, Lulu
Guo, Jingjing
Sánchez-Ferrer, Antoni
Shi, Xiangyan
Pervushin, Konstantin
Mu, Yuguang
Mezzenga, Raffaele
Miserez, Ali
Keywords: Materials Science and Engineering
Issue Date: 2019
Source: Hiew, S. H., Mohanram, H., Ling, L., Guo, J., Sánchez-Ferrer, A., Shi, X., . . ., Miserez, Ali. (2019). A Short Peptide Hydrogel with High Stiffness Induced by 3 10‐Helices to β‐Sheet Transition in Water. Advanced Science, 6(21), 1901173-. doi:10.1002/advs.201901173
Journal: Advanced Science
Abstract: Biological gels generally require polymeric chains that produce long-lived phys- ical entanglements. Low molecular weight colloids offer an alternative to macro- molecular gels, but often require ad-hoc synthetic procedures. Here, a short biomimetic peptide composed of eight amino acid residues derived from squid sucker ring teeth proteins is demonstrated to form hydrogel in water without any cross-linking agent or chemical modification and exhibits a stiffness on par with the stiffest peptide hydrogels. Combining solution and solid-state NMR, circular dichroism, infrared spectroscopy, and X-ray scattering, the peptide is shown to form a supramolecular, semiflexible gel assembled from unusual right-handed 310-helices stabilized in solution by π–π stacking. During gelation, the 310-helices undergo conformational transition into antiparallel β-sheets with formation of new interpeptide hydrophobic interactions, and molecular dynamic simulations corroborate stabilization by cross β-sheet oligomeriza- tion. The current study broadens the range of secondary structures available to create supramolecular hydrogels, and introduces 310-helices as transient building blocks for gelation via a 310-to-β-sheet conformational transition.
ISSN: 2198-3844
DOI: 10.1002/advs.201901173
DOI (Related Dataset):
Rights: © 2019 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Fulltext Permission: open
Fulltext Availability: With Fulltext
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