Please use this identifier to cite or link to this item:
Title: 3D double network (DN) hydrogel-based metastasis tumour model
Authors: Yew, Eugene Jin
Keywords: Science::Biological sciences::Molecular biology
Issue Date: 2020
Publisher: Nanyang Technological University
Abstract: The tumour microenvironment (TME) plays a significant role in cancer progression. Compared to biochemical signals, the influence of TME-derived biomechanical cues in the regulation of invasion and cancer metastasis remains unclear. Herein, a biomimetic collagen-alginate (CoAl) interpenetrating polymer network (IPN) with tuneable mechanical properties was developed to examine the effect of matrix stiffness on MDA-MB-231 invasiveness. Stiffness of the CoAl-IPN hydrogel with storage modulus (G’) ranging from 50-300 Pa can be attained by varying the crosslinking density of the alginate network. Increasing matrix stiffness leads to a build-up of mechanical stress and/or pressure experienced by the growing MDA-MB231 tumoroids as a result of the physical confinement effect. Interestingly, upon the selective degradation of the alginate network, tumoroids originally cultured in a “stiff” (~300 Pa) microenvironment displayed higher invasiveness in 3D compared to tumoroids grown in a “soft” (~100 Pa) CoAl-IPN. Specifically, the in situ characteristic starburst invasion projection of the tumoroids in the “stiff” group was found to be 4.3- fold larger relative to the “soft” experimental group. Therefore, using the developed 3D hybrid hydrogel culture system, the novel matrix-stiffness dependent mechanopriming of cancer cell invasion was revealed.
Schools: School of Biological Sciences 
Fulltext Permission: restricted
Fulltext Availability: With Fulltext
Appears in Collections:SBS Student Reports (FYP/IA/PA/PI)

Files in This Item:
File Description SizeFormat 
FYP Final.pdf
  Restricted Access
3D Double Network (DN) Hydrogel-Based Metastasis Tumour Model1.67 MBAdobe PDFView/Open

Page view(s) 50

Updated on Apr 22, 2024


Updated on Apr 22, 2024

Google ScholarTM


Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.