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Title: Fast-bactericidal effect of polyion complex nanoparticles on Gram-negative bacteria
Authors: Wei, Guangmin
Nguyen, Diep
Reghu, Sheethal
Li, Jianghua
Chua, Chun Song
Ishida, Yoshiki
Chan-Park, Mary Bee Eng
Keywords: Engineering::Chemical engineering
Issue Date: 2020
Source: Wei, G., Nguyen, D., Reghu, S., Li, J., Chua, C. S., Ishida, Y., & Chan-Park, M. B. (2020). Embargo fast-bactericidal effect of polyion complex nanoparticles on Gram-negative bacteria, 3(3), 2654-2664. doi:10.1021/acsanm.0c00010
Journal: ACS Applied Nano Materials
Abstract: There is an urgent need for effective bactericidal agents for use in real commercial formulations because many old disinfectants, such as halogenated compounds, are now banned. Cationic polymers may have good bactericidal properties in pure water or buffer but typically become ineffective in the presence of anionic surfactants that are widely used in many commercial formulations. Here, we discover that polyion complex (PIC) nanoparticles formed by cationic polymers of poly[3-(acrylamido)propyl]trimethylammonium chloride (PAMPTMA) in the presence of anionic surfactants display a promising fast-bactericidal effect (>99.99% killing within a 10 min treatment) on Gram-negative Escherichia coli (ATCC 8739). To examine the influence of the hydrophobicity on the bactericidal property, we synthesize PAMPTMA-b-poly(butyl methacrylate) and discover that increasing the hydrophobicity has little influence on the bactericidal property of PIC nanoparticles. A mechanism study shows that cationic PIC nanoparticles rapidly cause significant pores in both the outer and inner membranes because of their large size and high local concentration of positive charges. Rapid membrane pore formation results in fast cell death. The discovery—certain cationic polymers when formulated with anionic surfactants are even more bactericidal than neat cationic polymers alone—paves the way for potential applications of synthetic cationic polymers in commercial formulations.
ISSN: 2574-0970
DOI: 10.1021/acsanm.0c00010
Rights: This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Applied Nano Materials, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Journal Articles

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