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Title: Real‐time imaging of dynamic cell reprogramming with nanosensors
Authors: Wiraja, Christian
Yeo, David Chenloong
Tham, Khek-Chian
Chew, Sharon Wan Ting
Lim, Xinhong
Xu, Chenjie
Keywords: Engineering::Chemical engineering
Issue Date: 2018
Source: Wiraja, C., Yeo, D. C., Tham, K.-C., Chew, S. W. T., Lim, X., & Xu, C. (2018). Real‐time imaging of dynamic cell reprogramming with nanosensors. Small, 14(17), 1703440-. doi:10.1002/smll.201703440
Journal: Small
Abstract: Cellular reprogramming, the process by which somatic cells regain pluripotency, is relevant in many disease modeling, therapeutic, and drug discovery applications. Molecular evaluation of reprogramming (e.g., polymerase chain reaction, immunostaining) is typically disruptive, and only provides snapshots of phenotypic traits. Gene reporter constructs facilitate live-cell evaluation but is labor intensive and may risk insertional mutagenesis during viral transfection. Herein, the utilization of a non-integrative nanosensor is demonstrated to visualize key reprogramming events in situ within live cells. Principally based on sustained intracellular release of encapsulated molecular probes, nanosensors successfully monitored mesenchymal-epithelial transition, pluripotency acquisition, and transdifferentiation events. Tracking the dynamic expression of four pivotal biomarkers (i.e., THY1, E-CADHERIN, OCT4, and GATA4 mRNA), nanosensor signal showed great agreement with polymerase chain reaction and gene reporter imaging (R2 > 0.9). Overall, such facile, versatile nanosensor enables real-time monitoring of low-frequency reprogramming events, thereby useful for high-throughput assessment, optimization, and biomarker-specific cell enrichment.
ISSN: 1613-6810
DOI: 10.1002/smll.201703440
Rights: © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
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