Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/139188
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dc.contributor.authorSerra, Aidaen_US
dc.contributor.authorGallart-Palau, Xavieren_US
dc.contributor.authorDutta, Bamaprasaden_US
dc.contributor.authorSze, Siu Kwanen_US
dc.date.accessioned2020-05-18T02:47:07Z-
dc.date.available2020-05-18T02:47:07Z-
dc.date.issued2018-
dc.identifier.citationSerra, A., Gallart-Palau, X., Dutta, B., & Sze, S. K. (2018). Online removal of sodium dodecyl sulfate via weak cation exchange in liquid chromatography-mass spectrometry based proteomics. Journal of proteome research, 17(7), 2390-2400. doi:10.1021/acs.jproteome.8b00156en_US
dc.identifier.issn2193-5807en_US
dc.identifier.urihttps://hdl.handle.net/10356/139188-
dc.description.abstractBiological research often requires the use of sodium dodecyl sulfate (SDS) to solubilize protein samples; however, this detergent is not compatible with direct mass spectrometry (MS) analysis. Here, we report an online high-throughput proteomics method that permits standard in-solution digestion of SDS-containing samples followed by direct liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) analysis using weak cation-exchange chromatography (WCX). This approach, called the online removal of sodium dodecyl sulfate (Online reSDS), exploits the properties of WCX in a highly organic and mildly acidic medium to retain positively charged peptides by both hydrophilic interaction and electrostatic attraction while simultaneously repelling negative SDS molecules. This method was optimized to successfully analyze complex samples that contain up to 1% of SDS. Furthermore, online reSDS improves the identification of peptides with post-translational modifications (PTMs), such as deamidation and phosphorylation, without preliminary enrichment. In conclusion, we show that reSDS can facilitate research in proteomics by allowing the use of SDS in a wide range of LC-MS/MS applications with simplified sample-processing procedures.en_US
dc.description.sponsorshipMOE (Min. of Education, S’pore)en_US
dc.description.sponsorshipNMRC (Natl Medical Research Council, S’pore)en_US
dc.language.isoenen_US
dc.relation.ispartofJournal of proteome researchen_US
dc.rightsThis document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of proteome research, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.jproteome.8b00156en_US
dc.subjectScience::Biological sciencesen_US
dc.titleOnline removal of sodium dodecyl sulfate via weak cation exchange in liquid chromatography-mass spectrometry based proteomicsen_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.identifier.doi10.1021/acs.jproteome.8b00156-
dc.description.versionAccepted versionen_US
dc.identifier.pmid29888605-
dc.identifier.scopus2-s2.0-85048711654-
dc.identifier.issue7en_US
dc.identifier.volume17en_US
dc.identifier.spage2390en_US
dc.identifier.epage2400en_US
dc.subject.keywordsSodium Dodecyl Sulfateen_US
dc.subject.keywordsWeak Cation Exchangeen_US
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