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Title: Amyloid‑β peptide triggers membrane remodeling in supported lipid bilayers depending on their hydrophobic thickness
Authors: Meker, Sigalit
Chin, Hokyun
Sut, Tun Naw
Cho, Nam-Joon
Keywords: Engineering::Materials
Issue Date: 2018
Source: Meker, S., Chin, H., Sut, T. N., & Cho, N.-J. (2018). Amyloid-β peptide triggers membrane remodeling in supported lipid bilayers depending on their hydrophobic thickness. Langmuir, 34(32), 9548-9560. doi:10.1021/acs.langmuir.8b01196
Journal: Langmuir 
Abstract: Amyloid-β (Aβ) peptide has been implicated in Alzheimer's disease, which is a leading cause of death worldwide. The interaction of Aβ peptides with the lipid bilayers of neuronal cells is a critical step in disease pathogenesis. Recent evidence indicates that lipid bilayer thickness influences Aβ membrane-associated aggregation, while understanding how Aβ interacts with lipid bilayers remains elusive. To address this question, we employed supported lipid bilayer (SLB) platforms composed of different-length phosphatidylcholine (PC) lipids (C12:0 DLPC, C18:1 DOPC, C18:1-C16:0 POPC), and characterized the resulting interactions with soluble Aβ monomers. Quartz crystal microbalance-dissipation (QCM-D) experiments identified concentration-dependent Aβ peptide adsorption onto all tested SLBs, which was corroborated by fluorescence recovery after photobleaching (FRAP) experiments indicating that higher Aβ concentrations led to decreased membrane fluidity. These commonalities pointed to strong Aβ peptide-membrane interactions in all cases. Notably, time-lapsed fluorescence microscopy revealed major differences in Aβ-induced membrane morphological responses depending on SLB hydrophobic thickness. For thicker DOPC and POPC SLBs, membrane remodeling involved the formation of elongated tubule and globular structures as a passive means to regulate membrane stress depending on Aβ concentration. In marked contrast, thin DLPC SLBs were not able to accommodate extensive membrane remodeling. Taken together, our findings reveal that membrane thickness influences the membrane morphological response triggered upon Aβ adsorption.
ISSN: 0743-7463
DOI: 10.1021/acs.langmuir.8b01196
Rights: © 2018 American Chemical Society. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
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