Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/139281
Title: Incorporation of calcium sulfate dihydrate into hydroxyapatite microspheres to improve the release of bone morphogenetic protein-2 and accelerate bone regeneration
Authors: Baek, Jaeuk
Lee, Hyun
Jang, Tae-Sik
Song, Juha
Kim, Hyoun-Ee
Jung, Hyun-Do
Keywords: Engineering::Chemical engineering
Issue Date: 2018
Source: Baek, J., Lee, H., Jang, T.-S., Song, J., Kim, H.-E., & Jung, H.-D. (2018). Incorporation of calcium sulfate dihydrate into hydroxyapatite microspheres to improve the release of bone morphogenetic protein-2 and accelerate bone regeneration. ACS Biomaterials Science and Engineering, 4(3), 846-856. doi:10.1021/acsbiomaterials.7b00715
Journal: ACS Biomaterials Science and Engineering
Abstract: In this study, hydroxyapatite (HA)-based microspheres with the ability to deliver bone morphogenetic protein-2 (BMP-2) were developed for accelerating bone regeneration. The incorporation of calcium sulfate dihydrate (CSD) in the HA matrix improved the rate of BMP-2 release from the microspheres. Under physiological conditions, the CSD fully degraded within 7 days and generated pore channels in the microspheres. The porosity and pore size of the HA–CSD microspheres after CSD degradation were 34.3% ± 4.2% and 11.5 ± 2.4 μm, respectively, significantly larger than those of the HA microspheres (23.9% ± 3.1% and 8.7 ± 0.9 μm, respectively). The increased porosity directly affected the rate of BMP-2 release from the microspheres. An in vitro experiment showed that both the BMP-2 release rate and the total amount of BMP-2 released increased considerably when incorporating the HA microspheres with CSD. BMP-2 was released slowly from the HA microspheres for up to 6 weeks. BMP-2 release was notably improved in the HA–CSD biphasic microspheres compared to the microspheres without CSD; the rate of release was 2.4-times faster due to the pores created by CSD dissolution after 7 days. Prior to animal testing, in vitro cell tests were performed to evaluate the biocompatibility of the HA–CSD microspheres. During CSD dissolution, biocompatible bone-like apatite precipitated on the cell surfaces, and preosteoblasts grew on the microspheres. In vivo experiments using a rabbit lateral femoral condyle defect model demonstrated that the level of bone regeneration was significantly enhanced by mineralization on the surface, generated additional pores as well as improved BMP-2 release behavior. The HA–CSD microspheres accelerated new bone growth to fill the entire defect in 6 weeks, corresponding to a 170% improvement in performance compared to the HA microspheres.
URI: https://hdl.handle.net/10356/139281
ISSN: 2373-9878
DOI: 10.1021/acsbiomaterials.7b00715
Rights: © 2018 American Chemical Society. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:SCBE Journal Articles

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