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Title: Understanding the implications of engineered nanoparticle induced autophagy in human epidermal keratinocytes in vitro
Authors: Gautam, Archana
Rakshit, Moumita
Nguyen, Kim Truc
Kathawala, Mustafa Hussain
Nguyen, Luong Thi Hien
Tay, Chor Yong
Wong, Esther
Ng, Kee Woei
Keywords: Science::Biological sciences
Issue Date: 2019
Source: Gautam, A., Rakshit, M., Nguyen, K. T., Kathawala, M. H., Nguyen, L. T. H., Tay, C. Y., . . ., Ng, K. W. (2019). Understanding the implications of engineered nanoparticle induced autophagy in human epidermal keratinocytes in vitro. NanoImpact, 15, 100177-. doi:10.1016/j.impact.2019.100177
Journal: NanoImpact
Abstract: Engineered nanoparticles (NPs) such as TiO2 and ZnO are key UV-blocking ingredients in sunscreens. While toxicological risks of applying these materials are generally regarded as low due to minute levels of penetration across the skin, our understanding of the physiological influence of potential cell-nanoparticle interactions in the skin is limited. This study plugs the current knowledge gap by profiling TiO2 and ZnO NP interaction with primary human epidermal keratinocytes, based on potential levels of NP penetration across the skin. Specific attention was given to profiling real-life relevant levels of exposure, and accurate dosimetry measurements in vitro. ZnO was expectedly more cytotoxic than TiO2. Although both NPs generated Reactive Oxygen Species (ROS) and Mitochondrial Superoxide (MSO) within 4 h exposure to sub-lethal concentrations, ZnO induction of these oxidative stress markers increased much more significantly after 24 h exposure. Exposure to increasing NP concentrations increased autophagy induction along with activation of inflammatory responses in the keratinocytes, primarily through the TRAF6-mediated pathway. Sustained induction of autophagy led to degradation of TRAF6 and, only in the case of TiO2, reduced NF-κB activation. This pro-survival mode of autophagy induction provides further insights into the on-going debate on the use of these NPs in consumer products.
ISSN: 2452-0748
DOI: 10.1016/j.impact.2019.100177
Rights: © 2019 Elsevier B.V. All rights reserved. This paper was published in NanoImpact and is made available with permission of Elsevier B.V.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MSE Journal Articles

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