Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/139379
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dc.contributor.authorAsamitsu, Sefanen_US
dc.contributor.authorObata, Shunsukeen_US
dc.contributor.authorPhan, Anh Tuânen_US
dc.contributor.authorHashiya, Kaorien_US
dc.contributor.authorBando, Toshikazuen_US
dc.contributor.authorSugiyama, Hiroshien_US
dc.date.accessioned2020-05-19T05:53:08Z-
dc.date.available2020-05-19T05:53:08Z-
dc.date.issued2018-
dc.identifier.citationAsamitsu, S., Obata, S., Phan, A. T., Hashiya, K., Bando, T., & Sugiyama, H. (2018). Simultaneous binding of hybrid molecules constructed with dual DNA-binding components to a G-quadruplex and its proximal duplex. Chemistry - A European Journal, 24(17), 4428-4435. doi:10.1002/chem.201705945en_US
dc.identifier.issn0947-6539en_US
dc.identifier.urihttps://hdl.handle.net/10356/139379-
dc.description.abstractA G-quadruplex (quadruplex) is a nucleic acid secondary structure adopted by guanine-rich sequences and is considered to be relevant to various pharmacological and biological contexts. Although a number of researchers have endeavored to discover and develop quadruplex-interactive molecules, poor ligand designability originating from topological similarity of the skeleton of diverse quadruplexes has remained a bottleneck for gaining specificity for individual quadruplexes. This work reports on hybrid molecules that were constructed with dual DNA-binding components, a cyclic imidazole/lysine polyamide (cIKP), and a hairpin pyrrole/imidazole polyamide (hPIP), with the aim toward specific quadruplex targeting by reading out the local duplex DNA sequence adjacent to designated quadruplexes in the genome. By means of circular dichroism (CD), fluorescence resonance energy transfer (FRET), surface plasmon resonance (SPR), and NMR techniques, we showed the dual and simultaneous recognition of the respective segment via hybrid molecules, and the synergistic and mutual effect of each binding component that was appropriately linked on higher binding affinity and modest sequence specificity. Monitoring quadruplex and duplex imino protons of the quadruplex/duplex motif titrated with hybrid molecules clearly revealed distinct features of the binding of hybrid molecules to the respective segments upon their simultaneous recognition. A series of the systematic and detailed binding assays described here showed that the concept of simultaneous recognition of quadruplex and its proximal duplex by hybrid molecules constructed with the dual DNA-binding components may provide a new strategy for ligand design, enabling targeting of a large variety of designated quadruplexes at specific genome locations.en_US
dc.description.sponsorshipMOE (Min. of Education, S’pore)en_US
dc.language.isoenen_US
dc.relation.ispartofChemistry - A European Journalen_US
dc.rightsThis is the accepted version of the following article: Asamitsu, S., Obata, S., Phan, A. T., Hashiya, K., Bando, T., & Sugiyama, H. (2018). Simultaneous binding of hybrid molecules constructed with dual DNA-binding components to a G-quadruplex and its proximal duplex. Chemistry - A European Journal, 24(17), 4428-4435, which has been published in final form at dx.doi.org/10.1002/chem.201705945. This article may be used for non-commercial purposes in accordance with the Wiley Self-Archiving Policy [https://authorservices.wiley.com/authorresources/Journal-Authors/licensing/self-archiving.html].en_US
dc.subjectScience::Biological sciencesen_US
dc.titleSimultaneous binding of hybrid molecules constructed with dual DNA-binding components to a G-quadruplex and its proximal duplexen_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Physical and Mathematical Sciencesen_US
dc.identifier.doi10.1002/chem.201705945-
dc.identifier.pmid29380465-
dc.identifier.scopus2-s2.0-85042399427-
dc.identifier.issue17en_US
dc.identifier.volume24en_US
dc.identifier.spage4428en_US
dc.identifier.epage4435en_US
dc.subject.keywordsDual DNA-binding Componentsen_US
dc.subject.keywordsG-quadruplexesen_US
item.fulltextNo Fulltext-
item.grantfulltextnone-
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