Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/139528
Title: The effect of temporal manipulation of transforming growth factor beta 3 and fibroblast growth factor 2 on the derivation of proliferative chondrocytes from mensenchymal stem cells - a study monitored by quantitative reverse transcription polymerase chain reaction and molecular beacon based nanosensors
Authors: Tay, Li Min
Wiraja, Christian
Wu, Yingnan
Yang, Zheng
Lee, Eng Hin
Xu, Chenjie
Keywords: Engineering::Bioengineering
Issue Date: 2017
Source: Tay, L. M., Wiraja, C., Wu, Y., Yang, Z., Lee. E. H., & Xu, C. (2018). The effect of temporal manipulation of transforming growth factor beta 3 and fibroblast growth factor 2 on the derivation of proliferative chondrocytes from mensenchymal stem cells - a study monitored by quantitative reverse transcription polymerase chain reaction and molecular beacon based nanosensors. Journal of Biomedical Materials Research Part A, 106(4), 895-904. doi:10.1002/jbm.a.36286
Journal: Journal of Biomedical Materials Research Part A
Abstract: Proliferative chondrocytes are critical to realize regeneration of damaged epiphyseal growth plate. However, acquiring autologous replacement cells involves highly invasive procedures and often results in limited cell quantity. Mesenchymal stem cells (MSCs) are a potential source of chondrogenic cells for the treatment of cartilage disorders and injuries. The temporal effect of transforming growth factor beta 3 (TGFβ3) and fibroblast growth factor 2 (FGF2) on the derivation of proliferative chondrocytes from MSCs in three-dimensional agarose was investigated by manipulating the duration of TGFβ3 and FGF2 treatment. The differentiation process was monitored by quantitative reverse transcription polymerase chain reaction (qRT-PCR) as well as nanosensors containing two molecular beacons that target critical biomarkers for proliferative chondrocytes (i.e., collagen type-II messenger ribonucleic acid [mRNA] and Ki67 mRNA). The molecular beacon-based nanosensors were found to be comparable to qRT-PCR in measuring mRNA expression and thus providing a noninvasive mean to screen and monitor culture samples.
URI: https://hdl.handle.net/10356/139528
ISSN: 1549-3296
DOI: 10.1002/jbm.a.36286
Rights: © 2017 Wiley Periodicals, Inc. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:IGS Journal Articles

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